Evaluation of Transport Media and Specimen Transport Conditions for the Detection of SARS-CoV-2 by Use of Real-Time Reverse Transcription-PCR | Journal of Clinical Microbiology

DOI: 10.1128/JCM.00708-20

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TABLE 1
Stability of room temperature SARS-CoV-2 RNA detected by the Quest EUA rRT-PCR^a
↵a Testing was performed at QDID. Five replicates were assayed by rRT-PCR under the indicated conditions. UTM, medium for clinical specimens containing viruses, chlamydiae, mycoplasma, or ureaplasma; UTM-RT, universal transport medium for viruses, mycoplasma, and ureaplasma.
↵b N1 and N3 TaqMan probes were as described in the Quest Diagnostics EUA package insert.
TABLE 2
Stability of refrigerated SARS-CoV-2 RNA detected by the Quest EUA rRT-PCR^a
↵a Testing was performed at QDID. Five replicates were assayed by rRT-PCR under the indicated conditions.
↵b N1 and N3 TaqMan probes were as described in the Quest Diagnostics EUA package insert.
↵c Increase of >2 C_Ts from day 0.
TABLE 3
Stability of frozen SARS-CoV-2 RNA detected by the Quest EUA rRT-PCR^a
↵a Testing was performed at QDID. Five replicates were assayed by rRT-PCR under the indicated conditions.
↵b N1 and N3 TaqMan probes were as described in the Quest Diagnostics EUA package insert.
↵c Increase of >2 C_Ts from day 0.
↵d One of five replicates at day 5 did not yield detectable RNA for the N3 target.
TABLE 4
Additional Stability of SARS-CoV-2 RNA in Saline detected by the Quest EUA and Roche cobas EUA^a
↵a Testing was performed at Quest Diagnostics, Marlborough, MA. Three replicates were assayed by rRT-PCR at each of the indicated conditions.
↵b N1 and N3 TaqMan probes were as described in the Quest Diagnostics EUA package insert, and ORF1a and ORF1b (ORF1a/b) and E gene probes were as described in the Roche Diagnostics EUA package insert.
↵c Increase of >2 C_Ts from day 0.

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transport conditions

transport media

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