Special Issue Virology

Multicenter Evaluation of the Cepheid Xpert Xpress SARS-CoV-2 Test

Michael J. Loeffelholz, David Alland, Susan M. Butler-Wu, Utsav Pandey, Carlo Frederico Perno, Alice Nava, Karen C. Carroll, Heba Mostafa, Emma Davies, Ashley McEwan, Jennifer L. Rakeman, Randal C. Fowler, Jean-Michel Pawlotsky, Slim Fourati, Sukalyani Banik, Padmapriya P. Banada, Shobha Swaminathan, Soumitesh Chakravorty, Robert W. Kwiatkowski, Victor C. Chu, JoAnn Kop, Rajiv Gaur, Mandy L. Y. Sin, Duy Nguyen, Simranjit Singh, Na Zhang, David H. Persing
Alexander J. McAdam, Editor
DOI: 10.1128/JCM.00926-20

Article Figures & Data

Figures

  • FIG 1
    FIG 1

    Distribution of Xpert Xpress SARS-CoV-2 CT values in concordant positive specimens. Twelve specimens inconclusive by the New York EUA method and 21 specimens tested at Rutgers University Hospital are not included because the EUA version of the Xpert test was used (no RdRp target). The box center lines are medians. The boxes represent upper and lower quartiles. The bars represent minimum and maximum values, except for RdRp NPS/OPS and RdRp TA, where the maximum values are outliers. *, P < 0.0001 for comparison with the E target; **, P = 0.005 for comparison with the E target.

Tables

  • TABLE 1

    Limits of detection of the Xpert Xpress SARS-CoV-2 test using SARS-CoV-2 in nasopharyngeal-swab clinical matrix

    TABLE 1
  • TABLE 2

    Agreement of Xpert Xpress SARS-CoV-2 test and comparator RT-PCR tests

    TABLE 2

    • ↵a Pos, positive; Neg, negative.

    • ↵b PPA, positive percent agreement {[Pos/Pos ÷ (Pos/Pos + Neg/Pos)] × 100}.

    • ↵c NPA, negative percent agreement {[Neg/Neg ÷ (Neg/Neg + Pos/Neg)] × 100}.

  • TABLE 3

    Specimens with discrepant test results

    TABLE 3
  • TABLE 4

    Distribution of Xpert Xpress SARS-CoV-2 positive results by assay target reactivity

    TABLE 4

    • ↵a Twelve specimens were inconclusive by the New York EUA method, and 21 specimens tested at Rutgers University Hospital are not included because the EUA version of the Xpert test was used (no RdRp target). Both E and N2 targets were detected by the Xpert EUA test in 10 of these specimens, and the N2 target alone and E target alone were each detected in 1 specimen.

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KEYWORDS

SARS-CoV-2
COVID-19
Xpert
RT-PCR

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