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Journal of Clinical Microbiology
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Special Issue Virology

Influence of Different Inactivation Methods on Severe Acute Respiratory Syndrome Coronavirus 2 RNA Copy Number

Hailong Chen, Rui Wu, Yuan Xing, Quanli Du, Zerun Xue, Yanli Xi, Yujie Yang, Yangni Deng, Yuewen Han, Kaixin Li, Yang Luan, Yalan Zhang, Xiaoguang Wei, Tongtong Yu, Hao Li, Lingxiang Zhu, Shisheng Su, Hao Lian, Linping Lu, Chianru Tan, Haichao Zheng, Baozhong Chen, Pengbo Yu, Yong Guo, Chaofeng Ma
Alexander J. McAdam, Editor
Hailong Chen
aXi’an Center for Disease Control and Prevention, Xi’an, Shaanxi, China
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Rui Wu
aXi’an Center for Disease Control and Prevention, Xi’an, Shaanxi, China
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Yuan Xing
bXi’an Medical University, Xi’an, Shaanxi, China
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Quanli Du
aXi’an Center for Disease Control and Prevention, Xi’an, Shaanxi, China
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Zerun Xue
aXi’an Center for Disease Control and Prevention, Xi’an, Shaanxi, China
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Yanli Xi
aXi’an Center for Disease Control and Prevention, Xi’an, Shaanxi, China
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Yujie Yang
aXi’an Center for Disease Control and Prevention, Xi’an, Shaanxi, China
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Yangni Deng
aXi’an Center for Disease Control and Prevention, Xi’an, Shaanxi, China
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Yuewen Han
aXi’an Center for Disease Control and Prevention, Xi’an, Shaanxi, China
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Kaixin Li
aXi’an Center for Disease Control and Prevention, Xi’an, Shaanxi, China
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Yang Luan
aXi’an Center for Disease Control and Prevention, Xi’an, Shaanxi, China
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Yalan Zhang
aXi’an Center for Disease Control and Prevention, Xi’an, Shaanxi, China
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Xiaoguang Wei
aXi’an Center for Disease Control and Prevention, Xi’an, Shaanxi, China
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Tongtong Yu
aXi’an Center for Disease Control and Prevention, Xi’an, Shaanxi, China
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Hao Li
aXi’an Center for Disease Control and Prevention, Xi’an, Shaanxi, China
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Lingxiang Zhu
cNational Research Institute for Family Planning, Beijing, China
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Shisheng Su
dDepartment of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China
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Hao Lian
eTargetingOne Corporation, Beijing, China
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Linping Lu
eTargetingOne Corporation, Beijing, China
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Chianru Tan
dDepartment of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China
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Haichao Zheng
aXi’an Center for Disease Control and Prevention, Xi’an, Shaanxi, China
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Baozhong Chen
aXi’an Center for Disease Control and Prevention, Xi’an, Shaanxi, China
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Pengbo Yu
fShaanxi Center for Disease Control and Prevention, Xi’an, Shaanxi, China
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Yong Guo
dDepartment of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China
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Chaofeng Ma
aXi’an Center for Disease Control and Prevention, Xi’an, Shaanxi, China
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  • ORCID record for Chaofeng Ma
Alexander J. McAdam
Boston Children's Hospital
Roles: Editor
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DOI: 10.1128/JCM.00958-20
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    FIG 1

    Detection of SARS-CoV-2 ORF 1ab and N gene in the original samples using RT-qPCR (A) and dPCR (B). The results of RT-qPCR were expressed in cycle threshold (CT) values. The solid lines show the linear regression of the N gene copy number against the ORF 1ab copy number. The dashed lines indicate the 95% confidence intervals of the regression line.

  • FIG 2
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    FIG 2

    Comparison of RNA copy numbers (N gene and ORF 1ab) between the original group and the TRIzol treatment group (Tri). Data are shown as the mean ± SEM.

  • FIG 3
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    FIG 3

    Comparison of RNA copy numbers (N gene and ORF 1ab) between the original group and the 56°C 30 min treatment group. Data are shown as the mean ± SEM.

  • FIG 4
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    FIG 4

    Comparison of RNA copy numbers (N gene and ORF 1ab) between the 80°C 20 min (A), 100°C 20 min (B), and 121°C 20 min (C) treatment groups and the original group. Data are shown as the mean ± SEM.

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Influence of Different Inactivation Methods on Severe Acute Respiratory Syndrome Coronavirus 2 RNA Copy Number
Hailong Chen, Rui Wu, Yuan Xing, Quanli Du, Zerun Xue, Yanli Xi, Yujie Yang, Yangni Deng, Yuewen Han, Kaixin Li, Yang Luan, Yalan Zhang, Xiaoguang Wei, Tongtong Yu, Hao Li, Lingxiang Zhu, Shisheng Su, Hao Lian, Linping Lu, Chianru Tan, Haichao Zheng, Baozhong Chen, Pengbo Yu, Yong Guo, Chaofeng Ma
Journal of Clinical Microbiology Jul 2020, 58 (8) e00958-20; DOI: 10.1128/JCM.00958-20

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Influence of Different Inactivation Methods on Severe Acute Respiratory Syndrome Coronavirus 2 RNA Copy Number
Hailong Chen, Rui Wu, Yuan Xing, Quanli Du, Zerun Xue, Yanli Xi, Yujie Yang, Yangni Deng, Yuewen Han, Kaixin Li, Yang Luan, Yalan Zhang, Xiaoguang Wei, Tongtong Yu, Hao Li, Lingxiang Zhu, Shisheng Su, Hao Lian, Linping Lu, Chianru Tan, Haichao Zheng, Baozhong Chen, Pengbo Yu, Yong Guo, Chaofeng Ma
Journal of Clinical Microbiology Jul 2020, 58 (8) e00958-20; DOI: 10.1128/JCM.00958-20
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    • ABSTRACT
    • INTRODUCTION
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    • RESULTS
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KEYWORDS

COVID-19
inactivation
digital PCR
copy number

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