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Immunoassays

Detection of SARS-CoV-2 Antibodies in Oral Fluid Obtained Using a Rapid Collection Device

Melanie A. MacMullan, Prithivi Chellamuthu, Aubree Mades, Sudipta Das, Fred Turner, Vladimir I. Slepnev, Albina Ibrayeva
Yi-Wei Tang, Editor
Melanie A. MacMullan
aCurative Inc., San Dimas, California, USA
bMork Family Department of Chemical Engineering and Materials Science, Viterbi School of Engineering, University of Southern California, Los Angeles, California, USA
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Prithivi Chellamuthu
aCurative Inc., San Dimas, California, USA
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Aubree Mades
aCurative Inc., San Dimas, California, USA
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Sudipta Das
aCurative Inc., San Dimas, California, USA
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Fred Turner
aCurative Inc., San Dimas, California, USA
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Vladimir I. Slepnev
aCurative Inc., San Dimas, California, USA
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Albina Ibrayeva
aCurative Inc., San Dimas, California, USA
cEli and Edythe Broad Center for Regenerative Medicine & Stem Cell Research at USC, Department of Stem Cell Biology and Regenerative Medicine, W.M. Keck School of Medicine, University of Southern California, Los Angeles, California, USA
dDavis School of Gerontology, University of Southern California, Los Angeles, California, USA
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  • ORCID record for Albina Ibrayeva
Yi-Wei Tang
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DOI: 10.1128/JCM.02510-20
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ABSTRACT

Current commercially available methods for reliably detecting antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remain expensive and inaccessible due to the need for whole-blood collection by highly trained phlebotomists using personal protective equipment (PPE). We have evaluated an antibody detection approach using the OraSure Technologies oral antibody collection device (OACD) and their proprietary SARS-CoV-2 total antibody detection enzyme-linked immunosorbent assay (ELISA). We found that the OraSure test for total antibody detection in oral fluid had comparable sensitivity and specificity to commercially available serum-based ELISAs for SARS-CoV-2 antibody detection while allowing for a more accessible form of specimen collection with the potential for self-collection.

FOOTNOTES

    • Received 8 October 2020.
    • Returned for modification 31 October 2020.
    • Accepted 19 November 2020.
    • Accepted manuscript posted online 24 November 2020.
  • Supplemental material is available online only.

  • Copyright © 2021 American Society for Microbiology.

All Rights Reserved.

This article is made available via the PMC Open Access Subset for unrestricted noncommercial re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.

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Detection of SARS-CoV-2 Antibodies in Oral Fluid Obtained Using a Rapid Collection Device
Melanie A. MacMullan, Prithivi Chellamuthu, Aubree Mades, Sudipta Das, Fred Turner, Vladimir I. Slepnev, Albina Ibrayeva
Journal of Clinical Microbiology Jan 2021, 59 (2) e02510-20; DOI: 10.1128/JCM.02510-20

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Detection of SARS-CoV-2 Antibodies in Oral Fluid Obtained Using a Rapid Collection Device
Melanie A. MacMullan, Prithivi Chellamuthu, Aubree Mades, Sudipta Das, Fred Turner, Vladimir I. Slepnev, Albina Ibrayeva
Journal of Clinical Microbiology Jan 2021, 59 (2) e02510-20; DOI: 10.1128/JCM.02510-20
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KEYWORDS

ELISA
SARS-CoV-2
antibodies
oral fluid
rapid test
saliva
serology

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