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Mycology

Development of a Duplex Real-Time PCR Assay for the Differentiation of Blastomyces dermatitidis and Blastomyces gilchristii and a Retrospective Analysis of Culture and Primary Specimens from Blastomycosis Cases from New York (2005 to 2019)

Mitchell Kaplan, YanChun Zhu, Julianne V. Kus, Lisa McTaggart, Vishnu Chaturvedi, Sudha Chaturvedi
Kimberly E. Hanson, Editor
Mitchell Kaplan
aMycology Laboratory, Wadsworth Center, New York State Department of Health, Albany, New York, USA
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YanChun Zhu
aMycology Laboratory, Wadsworth Center, New York State Department of Health, Albany, New York, USA
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Julianne V. Kus
bPublic Health Laboratory, Public Health Ontario, Toronto, Ontario, Canada
cDepartment of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada
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  • ORCID record for Julianne V. Kus
Lisa McTaggart
bPublic Health Laboratory, Public Health Ontario, Toronto, Ontario, Canada
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Vishnu Chaturvedi
aMycology Laboratory, Wadsworth Center, New York State Department of Health, Albany, New York, USA
dDepartment of Biomedical Sciences, University at Albany, Albany, New York, USA
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Sudha Chaturvedi
aMycology Laboratory, Wadsworth Center, New York State Department of Health, Albany, New York, USA
dDepartment of Biomedical Sciences, University at Albany, Albany, New York, USA
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Kimberly E. Hanson
University of Utah
Roles: Editor
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DOI: 10.1128/JCM.02078-20
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ABSTRACT

Blastomycosis due to Blastomyces dermatitidis and Blastomyces gilchristii is a significant cause of respiratory mycoses in North America with occasional reported outbreaks. We developed a highly sensitive, specific, and reproducible TaqMan duplex real-time PCR assay for the differentiation of B. dermatitidis and B. gilchristii. The new assay permitted retrospective analysis of Blastomyces cultures (2005 to 2019) and primary clinical specimens from blastomycosis cases (2013 to 2019) from New York patients. We identified B. dermatitidis as the predominant pathogen in 38 cases of blastomycosis, while B. gilchristii was a minor pathogen involved in five cases; these findings expand understanding of blastomycosis in New York. The duplex real-time PCR assay could be implemented in reference and public health laboratories to further understand the ecology and epidemiology of blastomycosis due to B. dermatitidis and B. gilchristii.

FOOTNOTES

    • Received 7 August 2020.
    • Returned for modification 8 September 2020.
    • Accepted 1 December 2020.
    • Accepted manuscript posted online 9 December 2020.
  • Supplemental material is available online only.

  • Copyright © 2021 American Society for Microbiology.

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Development of a Duplex Real-Time PCR Assay for the Differentiation of Blastomyces dermatitidis and Blastomyces gilchristii and a Retrospective Analysis of Culture and Primary Specimens from Blastomycosis Cases from New York (2005 to 2019)
Mitchell Kaplan, YanChun Zhu, Julianne V. Kus, Lisa McTaggart, Vishnu Chaturvedi, Sudha Chaturvedi
Journal of Clinical Microbiology Feb 2021, 59 (3) e02078-20; DOI: 10.1128/JCM.02078-20

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Development of a Duplex Real-Time PCR Assay for the Differentiation of Blastomyces dermatitidis and Blastomyces gilchristii and a Retrospective Analysis of Culture and Primary Specimens from Blastomycosis Cases from New York (2005 to 2019)
Mitchell Kaplan, YanChun Zhu, Julianne V. Kus, Lisa McTaggart, Vishnu Chaturvedi, Sudha Chaturvedi
Journal of Clinical Microbiology Feb 2021, 59 (3) e02078-20; DOI: 10.1128/JCM.02078-20
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KEYWORDS

Blastomyces
ecology
epidemiology
molecular assay
validation

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