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Journal of Clinical Microbiology
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Mycology

Performance of Two Novel Chromogenic Media for the Identification of Multidrug-Resistant Candida auris Compared with Other Commercially Available Formulations

Auke W. de Jong, Chendo Dieleman, Mauricio Carbia, Ratna Mohd Tap, Ferry Hagen
Kimberly E. Hanson, Editor
Auke W. de Jong
aWesterdijk Fungal Biodiversity Institute, Utrecht, The Netherlands
bDepartment of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
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Chendo Dieleman
aWesterdijk Fungal Biodiversity Institute, Utrecht, The Netherlands
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Mauricio Carbia
cDepartamento de Parasitología y Micología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
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Ratna Mohd Tap
dMycology Laboratory, Institute for Medical Research, National Institute of Health, Shah Alam, Selangor, Malaysia
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Ferry Hagen
aWesterdijk Fungal Biodiversity Institute, Utrecht, The Netherlands
bDepartment of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
eLaboratory of Medical Mycology, Jining No. 1 People's Hospital, Jining, Shandong, People's Republic of China
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Kimberly E. Hanson
University of Utah
Roles: Editor
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DOI: 10.1128/JCM.03220-20
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ABSTRACT

Non-albicans Candida species are emerging in the nosocomial environment, with the multidrug-resistant (MDR) species Candida auris being the most notorious example. Consequently, rapid and accurate species identification has become essential. The objective of this study was to evaluate five commercially available chromogenic media for the presumptive identification of C. auris. Two novel chromogenic formulations, CHROMagar Candida Plus (CHROMagar) and HiCrome C. auris MDR selective agar (HiMedia), and three reference media, CandiSelect (Bio-Rad), CHROMagar Candida (CHROMagar), and Chromatic Candida (Liofilchem), were inoculated with a collection of 9 genetically diverse C. auris strains and 35 strains from closely related comparator species. After 48 h of incubation, the media were evaluated for their ability to detect and identify C. auris. All media had the same limitations in the differentiation of the more common species Candida dubliniensis and Candida glabrata. Only on CHROMagar Candida Plus did C. auris colonies develop a species-specific coloration. Nevertheless, the closely related pathogenic species Candida pseudohaemulonii and Candida vulturna developed a similar appearance as C. auris on this medium. CHROMagar Candida Plus was shown to be superior in the detection and identification of C. auris, with 100% inclusivity for C. auris compared to 0% and 33% for the reference media and HiCrome C. auris MDR selective agar, respectively. Although C. vulturna and C. pseudohaemulonii can cause false positives, CHROMagar Candida Plus was shown to be a valuable addition to the plethora of mostly molecular methods for C. auris detection and identification.

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Performance of Two Novel Chromogenic Media for the Identification of Multidrug-Resistant Candida auris Compared with Other Commercially Available Formulations
Auke W. de Jong, Chendo Dieleman, Mauricio Carbia, Ratna Mohd Tap, Ferry Hagen
Journal of Clinical Microbiology Mar 2021, 59 (4) e03220-20; DOI: 10.1128/JCM.03220-20

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Performance of Two Novel Chromogenic Media for the Identification of Multidrug-Resistant Candida auris Compared with Other Commercially Available Formulations
Auke W. de Jong, Chendo Dieleman, Mauricio Carbia, Ratna Mohd Tap, Ferry Hagen
Journal of Clinical Microbiology Mar 2021, 59 (4) e03220-20; DOI: 10.1128/JCM.03220-20
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KEYWORDS

Candida
chromogenic media
selective media
rapid identification
Candida auris
coinfection

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