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More articles from Minireview

  • Beyond Fever and Pain: Diagnostic Methods for Chikungunya Virus
    Minireview
    Beyond Fever and Pain: Diagnostic Methods for Chikungunya Virus

    Chikungunya virus (CHIKV) is an alphavirus that is primarily transmitted by Aedes species mosquitoes. Though reports of an illness consistent with chikungunya date back over 200 years, CHIKV only gained worldwide attention during a massive pandemic that began in East Africa in 2004.

    Muktha S. Natrajan, Alejandra Rojas, Jesse J. Waggoner
  • Understanding and Addressing CLSI Breakpoint Revisions: a Primer for Clinical Laboratories
    Minireview
    Understanding and Addressing CLSI Breakpoint Revisions: a Primer for Clinical Laboratories

    The Clinical and Laboratory Standards Institute (CLSI) has revised several breakpoints since 2010 for bacteria that grow aerobically. In 2019, these revisions include changes to the ciprofloxacin and levofloxacin breakpoints for the Enterobacteriaceae and Pseudomonas aeruginosa, daptomycin...

    Romney M. Humphries, April N. Abbott, Janet A. Hindler
  • A Systematic Review and Meta-analysis of the Diagnostic Accuracy of Nucleic Acid Amplification Tests for Tuberculous Meningitis
    Minireview
    A Systematic Review and Meta-analysis of the Diagnostic Accuracy of Nucleic Acid Amplification Tests for Tuberculous Meningitis

    The diagnosis of tuberculous meningitis (TBM) is difficult and poses a significant challenge to physicians worldwide. Recently, nucleic acid amplification (NAA) tests have shown promise for the diagnosis of TBM, although their performance has been variable.

    Ali Pormohammad, Mohammad Javad Nasiri, Timothy D. McHugh, Seyed Mohammad Riahi, Nathan C. Bahr
  • Open Access
    Phylogenomic Pipeline Validation for Foodborne Pathogen Disease Surveillance
    Minireview
    Phylogenomic Pipeline Validation for Foodborne Pathogen Disease Surveillance

    Foodborne pathogen surveillance in the United States is transitioning from strain identification using restriction digest technology (pulsed-field gel electrophoresis [PFGE]) to shotgun sequencing of the entire genome (whole-genome sequencing [WGS]). WGS requires a new suite of analysis tools, some of which have long histories in academia but are new to the field of public health and regulatory decision making.

    Ruth E. Timme, Errol Strain, Joseph D. Baugher, Steven Davis, Narjol Gonzalez-Escalona, Maria Sanchez Leon, Marc W. Allard, Eric W. Brown, Sandra Tallent, Hugh Rand
  • An Update on Mycobacterial Taxonomy, 2016–2017
    Minireview
    An Update on Mycobacterial Taxonomy, 2016–2017
    Nicole Parrish
  • Laboratory Diagnosis of Neonatal Herpes Simplex Virus Infections
    Minireview
    Laboratory Diagnosis of Neonatal Herpes Simplex Virus Infections

    Herpes simplex virus (HSV) is a common and often benign infection in humans; although it less commonly affects newborns, infection in this age group can be devastating. Newborns often present with nonspecific clinical findings, making timely and accurate diagnosis of infection critical.

    William J. Muller, Xiaotian Zheng
  • CRISPR-Cas Biology and Its Application to Infectious Diseases
    Minireview
    CRISPR-Cas Biology and Its Application to Infectious Diseases

    Infectious diseases remain a global threat contributing to excess morbidity and death annually, with the persistent potential for destabilizing pandemics. Improved understanding of the pathogenesis of bacteria, viruses, fungi, and parasites, along with rapid diagnosis and treatment of human infections, is essential for improving infectious disease outcomes worldwide.

    Jeffrey R. Strich, Daniel S. Chertow
  • Antimicrobial Susceptibility Testing for Polymyxins: Challenges, Issues, and Recommendations
    Minireview
    Antimicrobial Susceptibility Testing for Polymyxins: Challenges, Issues, and Recommendations

    Polymyxins, including polymyxin B and polymyxin E (colistin), are now increasingly being used worldwide to treat patients with multidrug-resistant (MDR) Gram-negative bacterial infections. This necessitates that laboratories employ an accurate and reliable method for the routine performance of polymyxin susceptibility testing.

    Fereshteh Ezadi, Abdollah Ardebili, Reza Mirnejad
  • Open Access
    Toward the Development of a Circulating Free DNA-Based <em>In Vitro</em> Diagnostic Test for Infectious Diseases: a Review of Evidence for Tuberculosis
    Minireview
    Toward the Development of a Circulating Free DNA-Based In Vitro Diagnostic Test for Infectious Diseases: a Review of Evidence for Tuberculosis

    The detection of circulating free DNA (cfDNA) has transformed the field of oncology and prenatal diagnostics. Clinical application of cfDNA for disease diagnosis and monitoring, however, is relatively recent in the field of infectious disease.

    B. Leticia Fernández-Carballo, Tobias Broger, Romain Wyss, Niaz Banaei, Claudia M. Denkinger
  • Open Access
    Genome-Based Prediction of Bacterial Antibiotic Resistance
    Minireview
    Genome-Based Prediction of Bacterial Antibiotic Resistance

    Clinical microbiology has long relied on growing bacteria in culture to determine antimicrobial susceptibility profiles, but the use of whole-genome sequencing for antibiotic susceptibility testing (WGS-AST) is now a powerful alternative. This review discusses the technologies that made this possible and presents results from recent studies to predict resistance based on genome sequences.

    Michelle Su, Sarah W. Satola, Timothy D. Read

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