Inactivating Zaire Ebolavirus in Whole Blood Thin Smears used for Malaria Diagnosis

Todd Cutts; Bradley Cook; Guillaume Poliquin; James Strong; Steven Theriault

doi:10.1128/JCM.02960-15

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Inactivating Zaire Ebolavirus in Whole Blood Thin Smears used for Malaria Diagnosis

Todd Cutts, Bradley Cook, Guillaume Poliquin, James Strong, Steven Theriault

Todd Cutts

1Applied Biosafety Research Program, Canadian Science Centre for Human and Animal Health and J. C. Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E 3P6, Canada and 745 Logan Street, Winnipeg, MB R3E 3L5, Canada

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Bradley Cook

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Guillaume Poliquin

3Office of the Scientific Director General, Canadian Science Centre for Human and Animal Health, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB, R3E 3P6

4Department of Pediatrics and Child Health, The University of Manitoba, Winnipeg, MB R3T 2N2, Canada

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James Strong

4Department of Pediatrics and Child Health, The University of Manitoba, Winnipeg, MB R3T 2N2, Canada

5Diagnostics Unit, Special Pathogens Program, Canadian Science Centre for Human and Animal Health, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E 3P6, Canada

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Steven Theriault

2Department of Microbiology, The University of Manitoba, Winnipeg, MB R3T 2N2, Canada

6Chief Applied Biosafety Research Program, Canadian Science Centre for Human and Animal Health and J. C. Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E 3P6, Canada and 745 Logan Street, Winnipeg, MB R3E 3L5, Canada

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For correspondence: Steven.Theriault@phac-aspc.gc.ca

DOI: 10.1128/JCM.02960-15

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ABSTRACT

Malaria is an important mimic or co-infection in potential Ebola virus disease patients. We evaluated the efficacy of 100% methanol inactivating Zaire ebolavirus Makona variant for malaria thin smear preparation. We determined that 100% methanol completely inactivated the virus after 15 minutes.

FOOTNOTES

↵*Corresponding Author. Steven.Theriault{at}phac-aspc.gc.ca 1015 Arlington St Winnipeg Manitoba R3E 3R2 204-784-7505

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Inactivating Zaire Ebolavirus in Whole Blood Thin Smears used for Malaria Diagnosis

Todd Cutts, Bradley Cook, Guillaume Poliquin, James Strong, Steven Theriault

Journal of Clinical Microbiology Feb 2016, JCM.02960-15; DOI: 10.1128/JCM.02960-15

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Inactivating Zaire Ebolavirus in Whole Blood Thin Smears used for Malaria Diagnosis

Todd Cutts, Bradley Cook, Guillaume Poliquin, James Strong, Steven Theriault

Journal of Clinical Microbiology Feb 2016, JCM.02960-15; DOI: 10.1128/JCM.02960-15

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Inactivating Zaire Ebolavirus in Whole Blood Thin Smears used for Malaria Diagnosis

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