RT Journal Article
SR Electronic
T1 Characterization of an 18-kilodalton Brucella cytoplasmic protein which appears to be a serological marker of active infection of both human and bovine brucellosis.
JF Journal of Clinical Microbiology
JO J. Clin. Microbiol.
FD American Society for Microbiology
SP 2141
OP 2145
VO 31
IS 8
A1 Goldbaum, F A
A1 Leoni, J
A1 Wallach, J C
A1 Fossati, C A
YR 1993
UL http://jcm.asm.org/content/31/8/2141.abstract
AB Some anticytoplasmic protein monoclonal antibodies (MAbs) from mice immunized by infection with Brucella ovis cells have been obtained. One of these MAbs, BI24, was used to purify by immunoaffinity a protein with a pI of 5.6 and a molecular mass of 18 kDa. This protein was present in all of the rough and smooth Brucella species studied, but it could not be detected in Yersinia enterocolitica 09. Three internal peptides of this protein were partially sequenced; no homology with other bacterial proteins was found. The immunogenicity of the 18-kDa protein was studied with both human and bovine sera by a capture enzyme-linked immunosorbent assay system with MAb BI24.