RT Journal Article
SR Electronic
T1 Development of a Nested PCR for Detection of<em>Cryptococcus neoformans</em> in Cerebrospinal Fluid
JF Journal of Clinical Microbiology
JO J. Clin. Microbiol.
FD American Society for Microbiology
SP 3438
OP 3440
VO 36
IS 11
A1 Rappelli, Paola
A1 Are, Riccardo
A1 Casu, Giuseppe
A1 Fiori, Pier Luigi
A1 Cappuccinelli, Piero
A1 Aceti, Antonio
YR 1998
UL http://jcm.asm.org/content/36/11/3438.abstract
AB We report the development of a nested-PCR-based assay for the detection of Cryptococcus neoformans in cerebrospinal fluid. The specificity and sensitivity of the test were assessed. The technique was then applied to 40 cerebrospinal fluid samples. We obtained positive reactions for all 21 clinical samples from patients who had been previously diagnosed as having cryptococcal meningitis by conventional techniques and negative reactions for all 19 negative controls. Nested PCR is here compared with other diagnostic methods currently used in patients’ follow-up exams during anticryptococcal therapy.