TABLE 1.

Oligonucleotide primers and detection probes for real-time PCR for the simultaneous detection of E. histolytica, G. lamblia, C. parvum and PhHV-1 (internal control)

Target organism and oligonucleotideOligonucleotide sequenceGenBank accession no. or reference
E. histolytica
    Ehd-239F5′-ATTGTCGTGGCATCCTAACTCA-3′ X64142 , Z49256
    Ehd-88R5′-GCGGACGGCTCATTATAACA-3′ X64142 , Z49256
    histolytica-96TVIC-5′-TCATTGAATGAATTGGCCATTT-3′-nonfluorescent quencher X64142
G. lamblia
    Giardia-80F5′-GACGGCTCAGGACAACGGTT-3′ M54878
    Giardia-127R5′-TTGCCAGCGGTGTCCG-3′ M54878
    Giardia-105TFAM-5′-CCCGCGGCGGTCCCTGCTAG-3′-black hole quencher 1 M54878
C. parvum
    CrF5′-CGCTTCTCTAGCCTTTCATGA-3′ 11
    CrR5′-CTTCACGTGTGTTTGCCAAT-3′ 11
    CryptoTexas Red-5′-CCAATCACAGAATCATCAGAATCGACTGGTATC-3′-black hole quencher 2 11
PhHV-1
    PhHV-267s5′-GGGCGAATCACAGATTGAATC-3′ 26
    PhHV-337as5′-GCGGTTCCAAACGTACCAA-3′ 26
    PhHV-305tqCy5-5′-TTTTTATGTGTCCGCCACCATCTGGATC-3′-black hole quencher 2 26