TABLE 4.

Comparison of AFLP with PCR ribotyping according to methods of Bidet and to O'Neill for 39 toxin A/B+ isolates of C. difficile and 12 unrelated isolates

Toxin profileIsolate designationSubmitting laboratory locationeAFLP typePRBa typePROb typetcdA deletion (kb)tcdB genecerm(B) gened
A/B+Can1Winnipeg20200171.8++
Can3Winnipeg20200171.8+
Can5Winnipeg20200171.8++
60Paris20200171.8++
8864Paris30300361.7+
98-15632Paris20200171.8++
98-16948Paris20200171.8++
98-4318Paris20200171.8++
99-3050Paris20200171.8++
Jap1Gifu20200171.8++
Jap2Gifu20200171.8+
Jap3Gifu20200171.8++
CD15Amsterdam20200171.8++
CD16Amsterdam20200171.8++
CD17Amsterdam20200171.8++
Ned6Leiden20200171.8++
Ned7Leiden20200171.8+
1110/98Warsaw20200171.8+
1745/00Warsaw20200171.8++
205/99Warsaw20200171.8++
2233/98Warsaw20200171.8++
2428/95Warsaw20200171.8++
2601/98Warsaw20200171.8++
2785/97Warsaw20200171.8++
2887/97Warsaw20200171.8++
399/98Warsaw20200171.8++
592/98Warsaw20200171.8++
P250/00Warsaw20200171.8++
P268/00Warsaw20200171.8++
CF2Chicago20200171.8+
CF4Chicago20200171.8++
R10205Cardiff20200171.8++
R10430Cardiff20200171.8++
R10542Cardiff20200471.8++
R11092-2Cardiff20b200171.8++
R12878Cardiff20200171.8++
R13044Cardiff20200171.8++
R13167Cardiff20200171.8++
R2140Cardiff20200171.8++
A+/B+98-15323Paris3232014−0.2+
98-15845Paris31310450.6+
CD21Amsterdam3333023None+
Ned2Leiden3434005None+
Ned3Leiden2422014None+
Ned4Leiden9b9018None++
Ned5Leiden9b9018None++
Ned8Leiden21b21001None+
R11092-1Cardiff2727110None+
R7771Cardiff2727110None+
R7981Cardiff2727110None+
A/BNed1Leiden35350852.5
  • a PRB, PCR ribotyping according to Bidet method.

  • b PRO, PCR ribotyping according to O'Neill method.

  • c PCR for detection of the tcdB gene.

  • d PCR for detection of the ermB gene.

  • e Submitting laboratories were located in Winnepeg, Canada; Paris, France; Gifu, Japan; Amsterdam, The Netherlands; Leiden, The Netherlands; Warsaw, Poland; Chicago, Ill.; and Cardiff, United Kingdom.