TABLE 1.

Sequences of primers used for detection of bla genes or genotyping of strains

ESBL or regionPrimerSequencePositionGenBank accession no. or reference
TEMTEM-A5′-ATA AAA TTC TTG AAG AC-3′1-17 X54604.1
TEM-B5′-TTA CCA ATG CTT AAT CA-3′1075-1059
SHVSHV-FOR5′-TGGTTATGCGTTATATTCGCC-3′69-89 X98100.1
SHV-REV5′-GCTTAGCGTTGCCAGTGCT-3′936-918
PRE-1PER-1-FOR5′-AATTTGGGCTTAGGGCAGAA-3′278-306 Z21957
PER-1-REV5′-ATGAATGTCATTATAAAAGC-3′1211-1192
VEB-1VEB-1-FOR5′-CGACTTCCATTTCCCGATGC-3′226-245 AF220758
VEB1-REV5′-GGACTCTGCAACAAATACGC-3′868-849
CTX-M-3M13U5′-GGTTAAAAAATCACTGCGTC-3′65-84 X92506
M13L5′-TTGGTGACGATTTTAGCCGC-3′928-909
CTX-M-9M9U5′-ATGGTGACAAAGAGAGTGCA-3′1-20 AF252621.2
M9L5′-CCC TTC GGC GAT GAT TCT C-3′870-852
CTX-M-2M25U5′-ATGATGACTCAGAGCATTCG-3′304-323 AJ416343.1
M25L5′-TGGGTTACGATTTTCGCCGC-3′1169-1150
VEBcasVEBcas-F5′-GTTAGCGGTAATTTAACCAGATAG-3′11-34 AY027870.1
VEBcas-B5′-CGGTTTGGGCTATGGGCAG-3′1081-1063
Rep-PCRERIC25′-AAGTAAGTGACTGGGGTGAGCG-3′ 22
REP 2-15′-ICG ICT TAT CIG GCC TAC-3′ 2
VEBINV15′-CAGTTTGAGCATTTGAATACAC-3′ 16
VEBINV25′-AGC GTA TTT GTT GCA GAG TCC-3′
Variable region of integron5-CS5′-GGC ATC CAA GCA GCA AGC-3′1163-1180 AF205943
3-CS5′-AAG CAG ACT TGA CCT GAT-3′9231-9214
VEB cloneVEBc-R5′-CCGGGATCCATGAAAATCGTAAAAAGGATATTATT-3′ AF220758
VEBc-L5′-CCCAAGCTTTTATTTATTCAAATAGTAATTCCACG-3