TABLE 2.

Structural analysis of Tn1546-like elements by overlapping PCR

Tn1546-like element typeNo. of isolatesPCR product with the following primer pairsa:
42-349164-921170-1891949-29761871-37263514-39783907-47943992-45114676-57695735-70358082-85058448-91388544-958010446-10577
BM41470++++++++++++++
I7++++++++++++++++
IIa1++++++++++++++++++
IIb7++++++++++++
IIc4++++++++
III1++++++++++++++++
  • a + and ++, amplicons the same size as and larger than those obtained for E. faecium BM4147, respectively; −, absence of an amplicon with the particular pair of primers. The numbers in each primer pair correspond to the first numbers of the positions listed in Table 1.