TABLE 3.

LightCycler quantification of C. albicans ERG11, CDR1, CDR2, and MDR1 mRNA

Isolate no.Susceptibility interpretationbMean expression units ± SDa
ERG11cCDR1dCDR2dMDR1
1R3.98 ± 0.644.89 ± 0.861.21 ± 0.160
2R5.39 ± 0.564.33 ± 0.761.71 ± 0.290.20 ± 0.03
3R4.78 ± 0.638.35 ± 0.160.85 ± 0.090.63 ± 0.16
4SDD3.90 ± 0.455.16 ± 0.442.01 ± 0.280
5SDD2.35 ± 0.4613.14 ± 1.431.01 ± 0.150.61 ± 0.14
6SDD3.97 ± 0.505.04 ± 0.602.82 ± 0.450
7SDD2.52 ± 0.402.42 ± 0.490.62 ± 0.04156.50 ± 11.63
8S2.08 ± 0.322.43 ± 0.830.26 ± 0.050
9S1.87 ± 0.081.43 ± 0.220.30 ± 0.020
10S1.56 ± 0.272.06 ± 0.220.24 ± 0.050
  • a Gene expression normalized to ACT1 expression for each isolate.

  • b Fluconazole susceptibility category (R, resistant; SDD, susceptible dose dependent; S, susceptible).

  • c Overexpression in R compared to S and SDD isolates and in SDD compared to S isolates (P ≤ 0.05).

  • d Overexpression in R compared to S isolates (P ≤ 0.08).