TABLE 1.

PCR and sequencing primers used in this study

Primer type and targetPrimer designationSequence
PCR primers
    fliCFli13GCGCGGAATAATGAGG CATAAAGC
Fli14GCTTTCGCTGCCTTGAT TGTGT
    fljBSense 56 (27)TGTCGATAACCTGGATG ACACAGG
Flj4 RGGCATCCAGTGTAGTA CCATTATC
    flpAFlp F2AGTCCCGTGGAGCCTT
Flp RCCGGATTAACGTATCA GAGACAGC
Sequencing primers
    External primers
        fliC allelesF15GAAATTCAGGTGCCGA TACAAGGG
F14sCGCTGCCTTGATTGTGT
        fljB allelesFTCGATAACCTGGATGA CACAG
RCATTTACAGCTATACAT TCCATAAAGA
    Internal primers
        All allelesFF1(CT)GAAAT(CT)AACAA CAACCTG
FF8GTTAGCAATCGCCTGAC CTG
        α cluster-specific1CAGATCAACTCTCAGAC CCTGG
2AACTGGGTGGCGTAGA( CT)GG(CT)AA
3TTTGCTGGCATTGTAGG TTTTAC
4TGGATGGTCAGGGTGT TGTC
5GTCTGCGCCACCCAGTT
6GTTCAACGGCGTGAAA GTCC
        G complex-specificG3CCATTAAAGGTGGTAA GGAAGGAG
G5ATCACCTTAGCAGGCA AAACC
G6GTAACATTCTTGAAGCT GGATTTC
G7AGTTTCGCACTCTCAT TTTTGG
        Z4 complex-specificZ4-1TTAGTTTTGATTCGGA TAAAGATGT
Z4-2GGCTTTTAGATCTGCT CCATC