TABLE 1.

Primers and probes used for RT-LightCycler PCR and Northern hybridization probe synthesis

GeneGenBank accession no.Primer or probedSequence (5′ to 3′)PCR amplicon size (bp)
ERG11 X13296 LC forwardTGG AGA CGT GAT GCT G204
LC reverseAGT ATG TTG ACC ACC CAT AA
Donor probeaAAT CTC TGC TAC TTA TAT GAA AGA AAT TAA ACT GAG
Acceptor probebGAG AAC GTG GTG ATA TTG ATC CAA AT
NH forwardcATT GGT ATT CTT ATG GGT GGT CAA CAT AC
NH reversecCCC AAT ACA TCT ATG TCT ACC ACC ACC
CDR1 X77589 Forward primerAAG AGA ACC ATT ACC AGG286
Reverse primerAGG AAT CGA CGG ATC AC
Donor probeCAA GAC CAG CAT CTC CAT ATA CTG TAT
Acceptor probeATT CTT TAT GCA AGT GAG GTA TGG TG
NH forwardGAG ATC TAC CCT TTA AGA TA
NH reverseGAA TCG GGA TTC AAT TG
CDR2 U63812 Forward primerATG CTG ATG CCC TAG T364
Reverse primerGCT TCC TTA GGA CAT GG
Donor probeATT GTT GTT ATT CTT AGA TGA ACC AAC TTC AG
Acceptor probeTTA GAT TCT CAA ACT GCC TGG TCG
NH forwardGGT ATA TAA ACT GGA CAA C
NH reverseGAA TCT GGG TCT AAT TGT
MDR1 X53823 Forward primerGGA GTT TAG GTG CTG T201
Reverse primerCGG TGA TGG CTC TCA A
Donor probeGCC AGT TGG AGA TGG ACT
Acceptor probeTTG GTT CAT GTG TAT CAT TTC TG
NH forwardcGAG TCG TAG CTA CAT TGC CAT TAA CA
NH reversecGGT GAT TTC TAA TGG TCT CCA TAA TGT
ACT1 X16377 Forward primerCCA GCT TTC TAC GTT TCC209
Reverse primerCTG TAA CCA CGT TCA GAC
Donor probeCGG TAT TGT TTT GGA TTC TGG TG
Acceptor probeTGG TGT TAC TCA CGT TGT TCC
NH forwardcACC GAA GCT CCA ATG AAT CCA AAA TCC
NH reversecGTT TGG TCA ATA CCA GCA GCT TCC AAA
  • a Donor probes were labeled at the 3′ end with fluorescein.

  • b Acceptor probes were labeled at the 5′ end with LightCyler Red 640.

  • c ACT, ERG11, and MDR1 Northern hybridization probe sequences were previously described (5).

  • d LC, LightCycler; NH, Northern hybridization.