TABLE 4.

Flagellar antigens not encoded by fliC

Expected antigenSerotype tested (formula)Sequence of fliC
z44Bulovka (I 6,7:z44:−)H:k homolog with a premature stop codon
Quinhon (I 47:z44:−)H:z homolog with a premature stop codon
z53IIIb 38:z53:z50H:l,v homolog with a 1,400-bp insertiona
IIIb 38:z53:−H:l,v homolog with a 1,400-bp insertiona
z60Aesch (I 6,8:z60:1,2)H:e,h homolog with a premature stop codon
z61IIIa 38:z61:−H:l,v homolog with a premature stop codon
IIIb 38:z61:z53H:l,v homolog with a premature stop codon
z71Delmenhorst (I 18:z71:−)H:z4,z23 homolog with an IS30 homolog insert
  • a Blast searches of GenBank release number 132 with the 1,400-nucleotide inserted sequence did not produce any significant matches, but a 152-amino-acid open reading frame from the insert had similarity to many putative transposases in GenBank. The best match was with a putative transposase from Pseudomonas putida (81% amino acid similarity; accession number AJ318529 [14a]).