TABLE 1.

Results of sequencing the QRDRs of 100 H. pylori isolates (65 Cips and 35 Cipr) and melting temperatures for each pair of hybridization probes (mutation probe 87 and mutation probe 91) depending on the genotype and phenotype

No. of strainsTriplet 87Triplet 91Phenotype (by Etest)Genotype (by real-time PCR)Tma (°C) with mutation probe:
8791
39AAC (Asn)GAT (Asp)CipsWild-type Cips57.0 ± 0.247.4 ± 0.2
26AAT (Asn)GAT (Asp)CipsWild-type Cips50.4 ± 0.254.7 ± 0.2
16AAA (Lys)GAT (Asp)CiprMutant Cipr48.7 ± 0.249.2 ± 0.2
8AAG (Lys)GAT (Asp)CiprMutant Cipr50.8 ± 0.251.5 ± 0.2
3AAT (Asn)GGT (Gly)CiprMutant Cipr50.4 ± 0.258.5 ± 0.2
3AAC (Asn)TAT (Tyr)CiprMutant Cipr57.0 ± 0.244.6 ± 0.2
2AAT (Asn)AAT (Asn)CiprMutant Cipr50.4 ± 0.252.0 ± 0.2
1AAC (Asn)AAT (Asn)CiprMutant Cipr57.0 ± 0.244.6 ± 0.2
2 mixed infectionsbAAC (Asn)GAT (Asp)CiprWild-type Cips57.0 ± 0.247.4 ± 0.2
AAA (Lys)GAT (Asp)Mutant Cipr48.7 ± 0.249.2 ± 0.2
  • a Tm, melting temperature. Melting temperatures can vary slightly between different runs, so the temperatures given are means ± standard deviations for more than 30 runs.

  • b By real-time PCR, two mixed isolates with Cips and Cipr isolates were detected.