TABLE 1.

Identification results with ID32C, VITEK 2, and ITS2-FLP

OrganismNo. of isolatesSystemNo. of isolates with the following result:Final identification established by:
IdentifiedLow discriminationMisidentificationUnidentified
C. albicans 7ID32C61 (C. sake)Green colonies on chromagar, morphology on cornmeal agar
VITEK 27
ITS2-FLP-I7
ITS2-FLP-BF7
C. dubliniensis 1ID32C1
VITEK 21Weak growth at 42°C
ITS2-FLP-I1
ITS2-FLP-BF1
C. glabrata 18ID32C171Pink colonies on chromagar, no pseudohyphae on cornmeal agar, T + M−a
VITEK 254 (C. magnoliae)3 (C. magnoliae)5Pink colonies on chromagar, no pseudohyphae on cornmeal agar, T + M−
1 (C. colliculosa)
ITS2-FLP-I18
ITS2-FLP-BF162
C. guilliermondii 3ID32C3
VITEK 23
ITS2-FLP-I3
ITS2-FLP-BF21
C. krusei 3ID32C3
VITEK 212Pink colonies on Chromagar plate
ITS2-FLP-I3
ITS2-FLP-BF3
C. parapsilosis 14ID32C14
VITEK 2122Morphology on cornmeal agar
ITS2-FLP-I131 (Trichosporon mucoides)Morphology on cornmeal agarb
ITS2-FLP-BF131 (T. mucoides)Morphology on cornmeal agarb
C. tropicalis 3ID32C3
VITEK 23
ITS2-FLP-I3
ITS2-FLP-BF3
C. neoformans 5ID32C5
VITEK 25
ITS2-FLP-I5
ITS2-FLP-BF32
B. capitatus 2ID32C2
VITEK 22
ITS2-FLP-I2
ITS2-FLP-BF2
S. cerevisae 4ID32C4
VITEK 231 (C. colliculosa)Purple colonies on chromagar, morphology on cornmeal agar
ITS2-FLP-I4
ITS2-FLP-BF4
C. palmioleophila 1ID32C1
VITEK 21
ITS2-FLP-I1 (C. guilliermondii)Sequencingc
ITS2-FLP-BF1 (C. guilliermondii)Sequencingc
Total61ID32C5812
VITEK 2417310
ITS2-FLP-I592
ITS2-FLP-BF5524
  • a T + M−, trehalose positive, maltose negative.

  • b Due to a software problem this strain was misidentified. This problem has been resolved, and similar misidentifications are no longer possible.

  • c C. palmioleophila and C. guilliermondii have identical ITS2 lengths (323 to 324 bp). Differentiation is possible by restriction with RsaI, which yields a 157-bp restriction fragment for C. guilliermondii and no restriction for C. palmioleophila.