TABLE 2.

Level of identification for each method (n = 316 isolates)

Group and genusa (no. of isolates)No. of isolates identified by:
16S rRNA gene sequencingConventional methods
Genus levelSpecies levelGenus levelSpecies levelUnable to identify or misidentified
Gram-positive isolates
    Anaerococcus (7)71700
    Clostridium (97)9793937113b
    Eggerthella (14)1414975
    Eubacterium (1)11001
    Finegoldia (3)32201
    Parvimonas (3)33300
    Peptoniphilus (8)81008
    Other (12)c1210517
Gram-negative isolates
    Bacteroides (129)12912412710226b
    Fusobacterium (15)151515140
    Parabacteroides (7)77752b
    Porphyromonas (1)11100
    Prevotella (11)11111084b
    Veillonella (4)43004
    Other (4)d43004
  • a Genus was defined by sequence-based identification. Conventional identification was considered correct to genus or species level using old nomenclature (e.g., Bacteroides species for Parabacteriodes species).

  • b Some anaerobes were correctly classified to genus level but misidentified to species level.

  • c Actinobaculum (n = 1), Catabacter (n = 1), Propionibacterium (n = 3), Ruminococcus (n = 3), Solobacterium (n = 2), and Tissierella (n = 2).

  • d Alistipes (n = 2), Biophila (n = 1), and Campylobacter (n = 1).