TABLE 3.

Representative misclassifications by conventional methods

GroupIdentification (no. of isolates) by:
Conventional methods16S rRNA gene sequencing
Gram-positive isolates Clostridium clostridioforme (3) Clostridium boltae (2), Clostridium hathewayi (1)
Clostridium perfringens (2) Clostridium baratii (1), Clostridium bifermentans (1)
Peptostreptococcus sp. (2) Veillonella parvula (1), Veillonella sp. (1)
Gram-negative isolates Parabacteroides distasonis (2) Bacteroides fragilis (1), Parabacteroides merdae (1)
Bacteroides theta/ovatus group (7)Novel Bacteroides sp. (3), Bacteroides caccae (1), Bacteroides intestinalis (1), Bacteroides uniformis (1), Clostridium hathewayi (1)
Bacteroides uniformis (3) Bacteroides intestinalis (2), Bacteroides ovatus (2)
Bacteroides vulgatus (8) Bacteroides caccae (1), Bacteroides dorei (4), Bacteroides finegoldii (1), Bacteroides fragilis (1), Eggerthella lenta (1)
Fusobacterium sp. (3) Clostridium symbiosum (2), Alistipes finegoldii (1)