TABLE 4.

RFLP analysis of the partial sequence of the fla gene of species from the Borrelia burgdorferi sensu lato complex

Speciesfla size (bp)In silico RFLP pattern for indicated restriction enzyme
CelIIDdeIHapIIHhaIHincII
B. burgdorferi B31488338, 150349, 139
B. afzelii VS461488380, 108305, 108, 42, 33
B. andersonii 21133488238, 150, 100
B. bissettii DN127487220, 117, 78, 72404, 83
B. californiensis 443a456a225, 117, 111, 3a281, 175a
B. carolinensis488365, 123221, 117, 78, 45, 27
B. carolinensis SCGT18488221, 117, 78, 72
B. garinii 20047488329, 78, 72, 9405, 83453, 35
B. japonica HO14488305, 150, 33348, 140
B. lusitaniae PotiB2488338, 150393, 83, 12453, 35
B. sinica CMN3488338, 150300, 105, 83453, 35
B. spielmanii A14S488338, 150348, 140
B. tanukii Hk501488338, 150256, 232
B. turdi Ya501488221, 150, 117405, 83453, 35
B. valaisiana VS116488188, 135, 117, 33, 15
  • a The partial flagellin sequence of B. californiensis available in GeneBank (accession number DQ393346) differs significantly in size and represents the region shifted 218 nt down to the 3′ end of the gene in comparison to the rest of the control species: i.e. its RFLP pattern is irrelevant.