TABLE 3.

RFLP analysis of the 5S-23S rRNA intergenic spacer of species from the Borrelia burgdorferi sensu lato complex

SpeciesAmplicon size (bp)In silico RFLP pattern for indicated restriction enzyme
DraIMseI
B. burgdorferi B31254144, 52, 29, 28107, 52, 38, 29, 28
B. afzelii VS461246174, 52, 20107, 68, 51, 20
B. andersonii 21133266No site119, 67, 52, 28
B. bissettii DN127257144, 53, 33, 27107, 52, 38, 33, 37
B. bissettii 25015253173, 53, 27107, 52, 34, 27, 17, 12, 4
B. californiensis 443253173, 80107, 51, 37, 30, 28
B. carolinensis253173, 53, 27107, 67, 52, 27
B. garinii 20047253201, 52107, 95, 51
B. garinii NT29253144, 57, 52107, 57, 51, 38
B. japonica HO14236No site107, 78, 51
B. lusitaniae PotiB2257145, 83, 29107, 82, 39, 29
B. sinica CMN3235157, 49, 29107, 48, 38, 29, 13
B. spielmanii A14S225158, 67108, 66, 51
B. tanukii Hk501245173, 52, 20174, 51, 20
B. turdi Ya501248144, 81, 23107, 51, 38, 21, 16, 8, 7
B. valaisiana VS116255203, 52174, 51, 23, 7