Optimal conditions described in this study to perform phenotypic detection of MBL production among P. aeruginosa, Acinetobacter spp., and Enterobacteriaceae clinical isolates

Isolate groupTestIMBLβ-Lactam substrateExpl conditiona
Acinetobacter spp.DDST5 μl MPA (1.4 mM)ImipenemDistance, 2.0 cm
P. aeruginosaDDST5 μl MPA (1.4 mM)CeftazidimeDistance, 2.0 cm
EnterobacteriaceaeCD10 μl EDTA (100 mM)ImipenemBreakpoint, 5 mm
  • a Breakpoint is the increase in the size of inhibition zone.