TABLE 1.

Effect of DNA background on the amplification of B. burgdorferi

DilutionaThreshold cycles (Ct) in various DNA backgroundsb
WaterHumanTickMouse
10−120.6, 20.7, 20.920.6, 20.3, 20.220.7, 20.6, 20.420.1, 20.4, 20.5
10−223.9, 23.7, 23.823.6, 23.8, 23.723.9, 23.7, 23.823.6, 23.7, 23.7
10−327.5, 27.7, 27.627.8, 27.2, 27.627.5, 27.2, 27.827.0, 27.3, 27.2
10−431.1, 31.1, 31.731.3, 31.8, 30.930.4, 30.6, 30.630.7, 31.4, 30.9
10−535.9, 36.3, 35.234.7, 34.6, 35.534.7, 34.4, 34.534.1, 34.0, 33.5
  • a Values represent the dilution factors using DNA extracted from B. burgdorferi strain B31 grown in BSK-H medium. The diluent for each dilution series consisted of undiluted DNA extracted from 200 μl of EDTA-treated whole blood (human), a single adult I. scapularis tick (tick), or 50 μl of EDTA-treated whole blood (mouse).

  • b Each value represents the threshold cycle from one of three separate real-time multiplex PCR amplifications.