TABLE 1.

Nucleotide sequences and positions of primers and probes

OligonucleotideaSequencePositionbProduct length (bp)
16S3 up5′-ATGCAAGTCGAGCGAAC-3′28-44282
16S3 down5′-TGTCTCAGTTCCAGTGTGGC-3′309-290
16S FITC probe5′-CACTTATAGATGGATCCGCGCCGCATTAGCTAGT-FITC-3′199-232
16S Red probe5′-Red-GTAAGGTAACGGCTTACCAAGGCAACGAT-Phos-3′235-263
  • a FITC, fluorescein isothiocyanate; Red, LightCycler-Red 640.

  • b Position of the nucleotide sequence in the S. epidermidis 16S rRNA gene (accession number L37605).