TABLE 2.

Enterovirus and IC primer and probe sequencesa

Primer or probeSequence
P1.3b5′-AATTCTAATACGACTCACTATAGGGCACCGGATGGCCAATCCA-3′
P2.2c5′-GATGCAAGGTCGCATATGAGGGTGTGAAGAGCCTATTGAG-3′
Wild-type EV-specific capture probed5′-biotin-CTCCGGCCCCTGAATGCGGCTAAT-3′
IC-specific capture probee5′-biotin-GCAAAGTATCATCCCTCCAG-3′
Generic ECL detection probef5′-ruthenium-labeled-GATGCAAGGTCGCATATGAG-3′
  • a Located in the 5′-NCR of the enteroviral genome.

  • b Underlining designates the overhang portion encoding the T7 RNA polymerase promoter. The primer is located at nucleotides 623 to 640 of V01150, poliovirus 1, strain Sabin 1.

  • c Underlining denotes the generic ECL detection probe sequence. The primer is located at nucleotides 413 to 432 of V01150, poliovirus 1, strain Sabin 1.

  • d The capture probe is located at nucleotides 446 to 469 of V01150, poliovirus 1, strain Sabin 1.

  • e A potato leaf roll virus sequence is substituted for the enterovirus sequence located between nucleotides 446 and 469 of V01150, poliovirus 1, strain Sabin 1.

  • f Supplied in the NucliSens basic kit.