TABLE 1.

Primers used for the construction of the enterovirus-specific external RNA standard and enterovirus-specific internal control

PrimerSequence
Polio-1 EcoRIa5′-GCGGAATTCTACCACCTCGGAATCTTCGA-3′
Polio-1 Csp45Ib5′-GCGTTCGAATAAACACACTCAATGGAGCG-3′
Entero/PLRV-Fc5′-GCAAAGTATCATCCCTCCAGCCCAACCTCGGGGCA-3′
Entero/PLRV-Rd5′-CTGGAGGGATGATACTTTGCGATTCTTATGTAGC-3′
T7PCRe5′-TAATACGACTCACTATAGGG-3′
Sp6PCRf5′-GATTTAGGTGACACTATAG-3′
  • a Upstream polio primer.

  • b Downstream polio primer.

  • c Downstream polio/PLRV primer.

  • d Upstream polio/PLRV primer.

  • e Upstream T7 PCR primer.

  • f Downstrean SP6 PCR primer.

  • g Boldface denotes the EcoRI restriction site, and underlining denotes the Csp45I restriction site.