TABLE 3.

Assay sensitivity

No. of samples testedEV iso inputaEV amp inputbEV ECL meancIC iso inputdIC amp inputeIC ECL mean
310010194,6824,0004001,039,194
310010132,7553,000300891,208
1210010174,7682,000200829,753
320020188,0564,000400687,146
320020244,8993,000300813,625
1220020245,3402,000200873,081
330030122,6564,000400973,012
330030424,4693,000300729,375
730030400,5632,000200747,121
  • a Total number of enterovirus RNA copies in 200 μl of CSF per isolation (iso).

  • b Total number of enterovirus RNA copies in 5 μl of eluate used per amplification reaction (amp).

  • c ECL mean RLU value for samples tested.

  • d Total number of enterovirus IC RNA copies added to CSF extraction.

  • e Total number of enterovirus IC RNA copies in 5 μl of eluate used per amplification reaction.