TABLE 1.

MGP sequences and the original GP5+/6+ sequencesa

PrimerFormer namebDirectionSequence, 5′-3′
GP5+ForwardTTTGTTACTGTGGTAGATACTAC
MGPAFAForwardACGTTGGATGTTTGTTACTGTGGTGGATACTAC
MGPBFBForwardACGTTGGATGTTTGTTACCGTTGTTGATACTAC
MGPCNewForwardACGTTGGATGTTTGTTACTAAGGTAGATACCACTC
MGPDFDForwardACGTTGGATGTTTGTTACTGTTGTGGATACAAC
MGP31NewForwardACGTTGGATGTTTGTTACTATGGTAGATACCACAC
GP6+ReverseGAAAAATAAACTGTAAATCATATTC
MGPGRGReverseACGTTGGATGGAAAAATAAACTGTAAATCATATTCCT
MGPHRHReverseACGTTGGATGGAAAAATAAATTGTAAATCATACTC
MGPIRIReverseACGTTGGATGGAAATATAAATTGTAAATCAAATTC
MGPJRJReverseACGTTGGATGGAAAAATAAACTGTAAATCATATTC
MGP18NewReverseACGTTGGATGGAAAAATAAACTGCAAATCATATTC
  • a Nucleotides that differ from the GP5+/6+ primers are in bold. All reverse primers are biotinylated at the 5′ end.

  • b For primers described by Söderlund-Strand et al. (20).