TABLE 1.

Primer and probe information

Primer/probeSource or referenceSequenceaTarget gene (position)b
Pbp-2b-JT-FThis study5′-CTC AGG TGG CTG TTA TTG C-3′pbp2b (614-632)
Pbp-2b-JT-RThis study5′-GCG CAA TGG AAG GTC CTA C-3′pbp2b (1996-2014)
LytA-F105′-ACG CAA TCT AGC AGA TGA AGC-3′lytA (327-347)
LytA-R105′-TGT TTG GTT GGT TAT TCG TGC-3′lytA (407-427)
LytA-probe10FAM 5′-TTT GCC GAA AAC GCT TGA TAC AGG G-3′ TAMRAlytA (351-375)
Pbp-2b-F85′-ATT CTT GGT ATA CTC AGG CT-3′pbp2b (1280-1299)
Pbp-2b -RMODThis study5′-TGT TTG GAC CAT ATA GGT ATT-3′pbp2b (1348-1368)
Pbp-2b-R85′-GTT TGG ACC ATA TAG GTA TTT-3′pbp2b (1349-1369)
Pbp-2b-probe8VIC 5′-CAC AGC GGT CCA AGC TCT-3′ TAMRApbp2b (1317-1334)
F2This study5′-TGT TCC AGG TTC GGT TGT-3′pbp2b (1161-1178)
R4This study5′-GAC TCT AGA TTG CTG GTG C-3′pbp2b (1415-1433)
  • a FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.

  • b S. pneumoniae R6 (GenBank no. AE008520).