TABLE 2.

Correlation of multiplex PCR and conventional serotyping results for Listeria tested strains

SpeciesSerovarNo. of strainsMultiplex PCR fragment amplificationMultiplex PCR serovar (sv) group classification
lmo1118 (906 bp)lmo0737 (691 bp)ORF2110 (597 bp)ORF2819 (471 bp)prs (370 bp)
L. monocytogenes 1/2a40a++ L. monocytogenes sv 1/2a, 3a
1/2b40++ L. monocytogenes sv 1/2b, 3b, 7
1/2c40+++ L. monocytogenes sv 1/2c, 3c
4b40+++ L. monocytogenes sv 4b, 4d, 4e
3a5++ L. monocytogenes sv 1/2a, 3a
3b5++ L. monocytogenes sv 1/2b, 3b, 7
3c5+++ L. monocytogenes sv 1/2c, 3c
75++ L. monocytogenes sv 1/2b, 3b, 7
4d5+++ L. monocytogenes sv 4b, 4d, 4e
4e5+++ L. monocytogenes sv 4b, 4d, 4e
4a5+ Listeria species
4c5+ Listeria species
L. innocua 6a5+ Listeria species
6b5+ Listeria species
L. ivanovii 58+ Listeria species
L. seeligeri 1/2b2+ Listeria species
L. welshimeri 4c1+ Listeria species
6a1+ Listeria species
  • a The L. monocytogenes serovar 1/2a EGDe strain, in which the lmo1118 gene was first identified, was the only strain from the serovar group 1/2a tested to amplify the 906-bp lmo1118 fragment.