TABLE 1.

Overview of the 246 strains evaluated in this study

Strain(s)aK serotype(s)bC patternSpeciescSource 1eSource 2fDate(s)
Strains
    SB165, CIP 52.2081C1a K. p. subsp. pneumoniaeK1 ref. strainA 5054
    CIP A2151C1a K. p. subsp. pneumoniaeEyelidFrance, France1954
    CIP 52.1441C1a K. p. subsp. pneumoniaeLaboratory strain
    3981C1a K. p. subsp. pneumoniaeSputumLouvain, Belgium1976
    4081C1a K. p. subsp. pneumoniaeBloodParis, France1976
    4281C1a K. p. subsp. pneumoniaeBloodSt Etienne, France1976
    4401C1a K. p. subsp. pneumoniaeBloodParis, France1977
    4671C1a K. p. subsp. pneumoniaeLaboratory strainLyon, France1977
    4681C1a K. p. subsp. pneumoniaeTracheaParis, France1977
    5031C1a K. p. subsp. pneumoniaeSputumRoanne, France1977
    6431C1a K. p. subsp. pneumoniaeCSFParis, France1980
    6871C1a K. p. subsp. pneumoniaeCSFCréteil, France1980
    7441C1a K. p. subsp. pneumoniaeMare, metritisParis, France1981
    7981C1a K. p. subsp. pneumoniaeSputumParis, France1982
    9201C1a K. p. subsp. pneumoniaePleural fluidPontarlier, France1984
    9261C1a K. p. subsp. pneumoniaeBloodParis, France1984
    SB166, CIP 52.1452C2a K. p. subsp. pneumoniaeK2 ref. strainB 5055
    6752C2a K. p. subsp. pneumoniaeVaccine strainMontpellier, France1980
    15942C2a K. p. subsp. pneumoniaeMonkeyMulhouse, France1988
    15842C2b K. p. subsp. pneumoniaeBloodBriançon, France1988
    7772C2c K. p. subsp. pneumoniaeBloodParis, France1981
    342C2d K. p. subsp. pneumoniaeLaboratory strain
    CIP 52.2042C2e K. p. subsp. pneumoniaeLaboratory strain
    352C2e K. p. subsp. pneumoniaeLaboratory strain
    7782C2e K. p. subsp. pneumoniaeCSFParis, France1981
    7792C2e K. p. subsp. pneumoniaeAbcessParis, France1981
    SB167, CIP 52.1463C3a K. p. subsp. pneumoniaeK3 ref. strainC 5046
    CIP 52.210T, ATCC 13884T3C3a K. p. subsp. rhinoscleromatisType strain
    CIP 82.91T, ATCC 13883T3C3a K. p. subsp. pneumoniaeType strain
    ATCC 69083C3a K. p. subsp. rhinoscleromatisReference strain
    CIP 54.453C3a K. p. subsp. rhinoscleromatisNoseVietnam1954
    CIP 54.1293C3a K. p. subsp. rhinoscleromatisRhinoscleromaVietnam1954
    4753C3a K. p. subsp. rhinoscleromatisSputumPoland1977
    5383C3a K. p. subsp. pneumoniaeUrineParis, France1977
    7453C3a K. p. subsp. rhinoscleromatisGranulomaAlgérie1981
    7463C3a K. p. subsp. rhinoscleromatisRhinoscleromaAlgeria1981
    8393C3a K. p. subsp. rhinoscleromatisNoseParis, France1982
    15093C3a K. p. subsp. pneumoniaeFecesParis, France1988
    16353C3a K. p. subsp. pneumoniaePusParis, France1989
    16493C3a K. p. subsp. pneumoniaeBloodParis, France1989
    34.813C3a K. p. subsp. rhinoscleromatisNoseFrance1981
    42.793C3a K. p. subsp. rhinoscleromatisNoseParis, France1979
    SB17853C3a K. p. subsp. rhinoscleromatisSputumPoland1977
    SB17933C3a K. p. subsp. rhinoscleromatisGranulomaWien, Austria2001
    SB168, CIP 52.211T4C4a K. p. subsp. ozaenaeK4 ref. strainD 5050
    ATCC 11296 T4C4a K. p. subsp. ozaenaeType strainIndonesia1930
    8634C4a K. p. subsp. ozaenaeSputumLyon, France1983
    15754C4a K. p. subsp. ozaenaeBloodParis, France1988
    12024C4a K. p. subsp. ozaenaeBloodParis, France1986
    15104C4a K. p. subsp. ozaenaeEar pusParis, France1988
    9194C4b K. p. subsp. ozaenaeLungTours, France1989
    9094C4c K. p. subsp. ozaenaeBronchial aspPontoise, France1984
    16114C4d K. p. subsp. ozaenaeBloodCaen, France1989
    7484C4e K. p. subsp. ozaenaeNoseAlgeria1981
    8894C4f K. p. subsp. ozaenaeBronchial aspGrenoble, France1983
    CIP 52.2125C5a K. p. subsp. ozaenaeK5 ref. strainE 5051
    1895C5a K. p. subsp. pneumoniaeLaboratory strain1973
    3255C5a K. p. subsp. pneumoniaeHumanParis, France1975
    4215C5a K. p. subsp. ozaenaeNoseKigali, Rwanda1976
    CIP 52.2136C6a K. p. subsp. pneumoniaeK6 ref. strainF 5052
    CIP 80.426C6b K. p. subsp. pneumoniaeWoodCompiègne, France1979
    SB179, CIP 52.2057C7a K. p. subsp. pneumoniaeK7 ref. strainAerogenes 4140
    15437C7a K. p. subsp. pneumoniaeHorse, penisParis, France1988
    15637C7a K. p. subsp. pneumoniaeHorse, penisParis, France1989
    15837C7a K. p. subsp. pneumoniaeHorseCaen, France1988
    16047C7a K. p. subsp. pneumoniaeHorse, spermParis, France1988
    CIP 52.2068C8a K. planticola K8 ref. strain Klebsiella 1015
    15748C8b K. p. subsp. pneumoniaeBloodParis, France1988
    SB181, CIP 52.2079C9a K. p. subsp. pneumoniaeK9 ref. strain Klebsiella 1056
    SB182, CIP 52.21410C10a K. p. subsp. pneumoniaeK10 ref. strain Klebsiella 919Source 1eSource 2fDate(s)
    114110C10b K. p. subsp. pneumoniaeBronchial aspLyon, France1986
    CIP 52.21511C11a K. p. subsp. pneumoniaeK11 ref. strain Klebsiella 390
    SB184, CIP 52.21612C12a K. p. subsp. pneumoniaeK12 ref. strain Klebsiella 313
    SB185, CIP 52.21713C13a K. p. subsp. pneumoniaeK13 ref. strain Klebsiella 1470
    SB186, CIP 52.21814C14a K. planticola K14 ref. strain138
    SB187, CIP 52.21915C15a K. p. subsp. pneumoniaeK15 ref. strainMich. 61
    SB188, CIP 52.22016C16a K. p. subsp. pneumoniaeK16 ref. strain2069/49
    163216C16a K. p. subsp. pneumoniaeSeptic tankParis, France1988
    SB189, CIP 52.22117C17a K. p. subsp. pneumoniaeK17 ref. strain2005/49
    SB190, CIP 52.22218C18a K. p. subsp. pneumoniaeK18 ref. strain1754/49
    SB191, CIP 52.22319C19a K. p. subsp. pneumoniaeK19 ref. strain293/50
    SB19220C20a K. p. subsp. pneumoniaeK20 ref. strain889/50
    SB193, CIP 52.22521C21a K. p. subsp. pneumoniaeK21 ref. strain1702/49
    SB194, CIP 52.22622C22,37a K. p. subsp. pneumoniaeK22 ref. strain1996/49
    SB195, CIP 52.22823C23a K. p. subsp. pneumoniaeK23 ref. strain2812/50
    SB196, CIP 52.22924C24a K. p. subsp. pneumoniaeK24 ref. strain1680/49
    158924C24a K. p. subsp. pneumoniaeBloodParis, France1988
    SB197, CIP 52.23025C25a K. p. subsp. pneumoniaeK25 ref. strain2002/49
    158125C25b K. p. subsp. pneumoniaeBloodParis, France1988
    160125C25b K. p. subsp. pneumoniaeFecesCréteil, France1988
    SB198, CIP 53.626C26a K. oxytoca K26 ref. strain5884
    SB199, CIP 52.23227C27a K. p. subsp. pneumoniaeK27 ref. strain6613
    SB200, CIP 52.23328C28a K. p. subsp. pneumoniaeK28 ref. strain5758
    SB317, CIP 52.23429C29a K. oxytoca K29 ref. strain5725y
    SB318, CIP 52.23530C30a K. p. subsp. pneumoniaeK30 ref. strain7824
    156930C30a K. p. subsp. pneumoniaeHorse, penisParis, France1988
    162330C30a K. p. subsp. pneumoniaeHorse, spermSaint Lo, France1988
    155330C30b K. p. subsp. pneumoniaeHorse, spermLyon, France1989
    SB319, CIP 52.23131C31a K. p. subsp. pneumoniaeK31 ref. strain6258
    SB33932C32a K. planticola K32 ref. strain6837
    SB473, CIP 53.833C33a K. p. subsp. pneumoniaeK33 ref. strain6168
    SB474, CIP 53.934C34a K. p. subsp. pneumoniaeK34 ref. strain7522
    SB475, CIP 53.1035C35a K. planticola K35 ref. strain7444
    151635C35b K. p. subsp. pneumoniaeUrineParis, France1988
    138635C35c K. p. subsp. pneumoniaeEnterocolitisMontpellier, France1987
    SB476, CIP 53.1136C36a K. p. subsp. pneumoniaeK36 ref. strain8306
    SB477, CIP 53.1237C22,37a K. p. subsp. pneumoniaeK37 ref. strain8238
    CIP 53.1338C38a K. p. subsp. pneumoniaeK38 ref. strain8414
    SB479, CIP 53.1439C39a K. planticola K39 ref. strain7749
    58539C39b K. p. subsp. pneumoniaeHandSt Etienne, France1979
    SB480, CIP 53.1540C40a K. p. subsp. pneumoniaeK40 ref. strain8588
    SB48141C41a K. p. subsp. pneumoniaeK41 ref. strain6177
    SB482, CIP 53.1742C42a K. p. subsp. pneumoniaeK42 ref. strain1702
    SB483, CIP 53.1943C43a K. p. subsp. pneumoniaeK43 ref. strain2482
    SB484, CIP 53.2044C44a K. planticola K44 ref. strain7730
    SB485, CIP 53.2145C45a K. p. subsp. pneumoniaeK45 ref. strain8464
    SB486, CIP 53.2246C46a K. p. subsp. pneumoniaeK46 ref. strain5281
    SB487, CIP 53.2347C47a K. p. subsp. pneumoniaeK47 ref. strain9682
    70047C47a K. p. subsp. pneumoniaeWood, tropicalCompiègne, France1980
    165947C47b K. p. subsp. pneumoniaeFecesParis, France1989
    SB488, CIP 53.2448C48a K. planticola K48 ref. strain1196
    SB489, CIP 52.19949C49a K. planticola K49 ref. strain6115
    CIP 52.20050C50a K. p. subsp. pneumoniaeK50 ref. strain1303/50
    SB491, CIP 52.20151C51a K. p. subsp. pneumoniaeK51 ref. strain4715/50
    SB49252C52a K. p. subsp. pneumoniaeK52 ref. strain5759/50
    MGH 7857852C52a K. p. subsp. pneumoniaeReference strain
    157952C52b K. omithinolytica Bronchial aspToulouse, France1988
    SB493, CIP 53.2653C53a K. planticola K53 ref. strain1756/51
    SB494, CIP 53.2754C54a K. planticola K54 ref. strainStanley
    SB49555C55a K. p. subsp. pneumoniaeK55 ref. strain3985/51
    SB49656C56a K. planticola K56 ref. strain3534/51
    SB49757C57a K. planticola K57 ref. strain4425/51
    SB49858C58a K. planticola K58 ref. strain636/52
    9459C59a K. oxytoca UnknownParis, France1972
    SB50060C60a K. p. subsp. pneumoniaeK60 ref. strain4463/52
    SB50161C61a K. p. subsp. pneumoniaeK61 ref. strain5710/52
    SB50262C62a K. p. subsp. pneumoniaeK62 ref. strain5711/52
    162962C62a K. p. subsp. pneumoniaeDonkeyMulhouse, France1988
    151362C62b K. p. subsp. pneumoniaeFecesParis, France1988
    19962C62c K. oxytoca Bronchial aspParis, France1974
    SB50363C63a K. p. subsp. pneumoniaeK63 ref. strain5845/52
    SB50464C64a K. p. subsp. pneumoniaeK64 ref. strainNCTC 8172
    SB50565C65a K. terrigena K65 ref. strainSW 4
    SB50666C66a K. oxytoca K66 ref. strain438 (3a)
    SB50767C67a K. p. subsp. pneumoniaeK67 ref. strain264 (1)
    118768C68a K. oxytoca WoundParis, France1986
    134468C68a K. oxytoca BloodBordeaux, France1986
    SB50869C69a K. p. subsp. pneumoniaeK69 ref. strain889
    SB50970C70a K. oxytoca K70 ref. strain167
    SB51071C71a K. planticola K71 ref. strain4349
    SB51172C72a K. planticola K72 ref. strain1205
    SB51274C74a K. oxytoca K74 ref. strain371
    SB51379C79a K. planticola K79 ref. strain325
    SB51480C80a K. p. subsp. pneumoniaeK80 ref. strain708
    SB51581C81a K. p. subsp. pneumoniaeK81 ref. strain370
    SB51682C82a K. p. subsp. pneumoniaeK82 ref. strain3454-70
    10371,58C1a K. p. subsp. pneumoniaeHorsePont l’Eveque, France1985
    10561,58C1a K. p. subsp. pneumoniaeHorseCaen, France1985
    15701,58C1a K. p. subsp. pneumoniaeMare, metritisParis, France1988
    60721,31C21,31a K. p. subsp. pneumoniaeUrineSaint-Etienne, France1978
    162222,37C22,37a K. p. subsp. ozaenaeSputumMali1989
    683AcapsularC2e K. p. subsp. pneumoniaeHumanLyon, France1980
    1340AcapsularC3a K. p. subsp. pneumoniaeBloodBordeaux, France1987
    156730C30a K. p. subsp. pneumoniaeHorse, penisParis, France1988
    1609AcapsularC14a K. p. subsp. pneumoniaeUrineParis, France1988
    SB49959No or weak PCR product K. planticola K59 ref. strain2212/52
    15862No or weak PCR product K. p. subsp. pneumoniaeBloodBriançon, France1988
    12836No or weak PCR product K. terrigena CarrotsMulhouse, France1988
    14166No or weak PCR product K. terrigena Horse, spermNantes, France1988
    1476AcapsularNo or weak PCR product K. p. subsp. pneumoniaeHospital env.Rennes, France1988
    148316No or weak PCR product K. p. subsp. pneumoniaeHospital env.Rennes, France1988
    148524No or weak PCR product K. p. subsp. pneumoniaeHospital env.Rennes, France1988
    15447No or weak PCR product K. p. subsp. pneumoniaeHorse, penisParis, France1988
    157868No or weak PCR product K. oxytoca BloodParis, France1988
    13469No unique PCR product K. p. subsp. pneumoniaeBloodBordeaux, France1987
    15476No unique PCR product K. p. subsp. pneumoniaeMare, uterusCaen, France1988
    15763No unique PCR product K. p. subsp. pneumoniaeUrineParis, France1988
    7754No unique PCR product K. p. subsp. ozaenaeNoseDakar, Senegal1981
Strains in challenge collection of clinical isolatesd
    SB5422, 37C22,37a K. p. subsp. pneumoniaeBloodSeville, Spain1997-1998
    SB5237C22,37a K. p. subsp. pneumoniaeBloodSeville, Spain1997-1998
    SB111124C24a K. p. subsp. pneumoniaeUrineCoimbra, Portugal1997-1998
    SB6425C25b K. p. subsp. pneumoniaeBloodLondon, United Kingdom1997-1998
    SB11827C27a K. p. subsp. pneumoniaeWoundAthens, Greece1997-1998
    SB13827C27a K. p. subsp. pneumoniaeWoundAthens, Greece1997-1998
    SB15330C30a K. p. subsp. pneumoniaeBloodAnkara, Turkey1997-1998
    SB16230C30a K. p. subsp. pneumoniaeBloodIstanbul, Turkey1997-1998
    SB22330C30a K. p. subsp. pneumoniaeBloodAnkara, Turkey1997-1998
    SB11430C30b K. p. subsp. pneumoniaeBloodAthens, Greece1997-1998
    SB59230C30b K. p. subsp. pneumoniaeBloodAthens, Greece1997-1998
    SB9339C39b K. p. subsp. pneumoniaeBloodKrakow, Poland1997-1998
    SB21052C52a K. p. subsp. pneumoniaeUrineLille, France1997-1998
    SB4462C62a K. p. subsp. pneumoniaeBloodUtrecht, The Netherlands1997-1998
    SB16435C-NEW-1 K. p. subsp. pneumoniaeBloodAthens, Greece1997-1998
    SB20422, 37CNEW-2 K. p. subsp. pneumoniaeBloodLondon, United Kingdom1997-1998
    SB120NTCNEW-3 K. p. subsp. pneumoniaeBloodGenoa, Italy1997-1998
    SB39NTCNEW-3 K. p. subsp. pneumoniaeBloodGenoa, Italy1997-1998
    SB11324C24alike-a K. p. subsp. pneumoniaeUrineLille, France1997-1998
    SB15868C68alike-a K. p. subsp. pneumoniaeUrineGenoa, Italy1997-1998
    03-91382C2a K. p. subsp. pneumoniaeAbcessFrance2003
    03-24553C3a K. p. subsp. rhinoscleromatisNoseMali2003
  • a Entries with two strain names separated by a comma correspond to the 47 duplicates (see Materials and Methods).

  • b NT, nontypeable; two numbers separated by a comma mean that the strain reacted with both antisera.

  • c K. p., K. pneumoniae.

  • d The K serotypes of the clinical strains were determined in this study after cps PCR-RFLP analysis.

  • e ref., reference; CSF, cerebrospinal fluid; asp, aspirate; env., environment.

  • f Where source 1 is a reference strain, source 2 is the original strain name in the reference collection of the WHO reference center for E. coli and Klebsiella.