TABLE 1.

Overview of the 246 strains evaluated in this study

Strain(s)aK serotype(s)bC patternSpeciescSource 1eSource 2fDate(s)
Strains
    SB165, CIP 52.2081C1aK. p. subsp. pneumoniaeK1 ref. strainA 5054
    CIP A2151C1aK. p. subsp. pneumoniaeEyelidFrance, France1954
    CIP 52.1441C1aK. p. subsp. pneumoniaeLaboratory strain
    3981C1aK. p. subsp. pneumoniaeSputumLouvain, Belgium1976
    4081C1aK. p. subsp. pneumoniaeBloodParis, France1976
    4281C1aK. p. subsp. pneumoniaeBloodSt Etienne, France1976
    4401C1aK. p. subsp. pneumoniaeBloodParis, France1977
    4671C1aK. p. subsp. pneumoniaeLaboratory strainLyon, France1977
    4681C1aK. p. subsp. pneumoniaeTracheaParis, France1977
    5031C1aK. p. subsp. pneumoniaeSputumRoanne, France1977
    6431C1aK. p. subsp. pneumoniaeCSFParis, France1980
    6871C1aK. p. subsp. pneumoniaeCSFCréteil, France1980
    7441C1aK. p. subsp. pneumoniaeMare, metritisParis, France1981
    7981C1aK. p. subsp. pneumoniaeSputumParis, France1982
    9201C1aK. p. subsp. pneumoniaePleural fluidPontarlier, France1984
    9261C1aK. p. subsp. pneumoniaeBloodParis, France1984
    SB166, CIP 52.1452C2aK. p. subsp. pneumoniaeK2 ref. strainB 5055
    6752C2aK. p. subsp. pneumoniaeVaccine strainMontpellier, France1980
    15942C2aK. p. subsp. pneumoniaeMonkeyMulhouse, France1988
    15842C2bK. p. subsp. pneumoniaeBloodBriançon, France1988
    7772C2cK. p. subsp. pneumoniaeBloodParis, France1981
    342C2dK. p. subsp. pneumoniaeLaboratory strain
    CIP 52.2042C2eK. p. subsp. pneumoniaeLaboratory strain
    352C2eK. p. subsp. pneumoniaeLaboratory strain
    7782C2eK. p. subsp. pneumoniaeCSFParis, France1981
    7792C2eK. p. subsp. pneumoniaeAbcessParis, France1981
    SB167, CIP 52.1463C3aK. p. subsp. pneumoniaeK3 ref. strainC 5046
    CIP 52.210T, ATCC 13884T3C3aK. p. subsp. rhinoscleromatisType strain
    CIP 82.91T, ATCC 13883T3C3aK. p. subsp. pneumoniaeType strain
    ATCC 69083C3aK. p. subsp. rhinoscleromatisReference strain
    CIP 54.453C3aK. p. subsp. rhinoscleromatisNoseVietnam1954
    CIP 54.1293C3aK. p. subsp. rhinoscleromatisRhinoscleromaVietnam1954
    4753C3aK. p. subsp. rhinoscleromatisSputumPoland1977
    5383C3aK. p. subsp. pneumoniaeUrineParis, France1977
    7453C3aK. p. subsp. rhinoscleromatisGranulomaAlgérie1981
    7463C3aK. p. subsp. rhinoscleromatisRhinoscleromaAlgeria1981
    8393C3aK. p. subsp. rhinoscleromatisNoseParis, France1982
    15093C3aK. p. subsp. pneumoniaeFecesParis, France1988
    16353C3aK. p. subsp. pneumoniaePusParis, France1989
    16493C3aK. p. subsp. pneumoniaeBloodParis, France1989
    34.813C3aK. p. subsp. rhinoscleromatisNoseFrance1981
    42.793C3aK. p. subsp. rhinoscleromatisNoseParis, France1979
    SB17853C3aK. p. subsp. rhinoscleromatisSputumPoland1977
    SB17933C3aK. p. subsp. rhinoscleromatisGranulomaWien, Austria2001
    SB168, CIP 52.211T4C4aK. p. subsp. ozaenaeK4 ref. strainD 5050
    ATCC 11296 T4C4aK. p. subsp. ozaenaeType strainIndonesia1930
    8634C4aK. p. subsp. ozaenaeSputumLyon, France1983
    15754C4aK. p. subsp. ozaenaeBloodParis, France1988
    12024C4aK. p. subsp. ozaenaeBloodParis, France1986
    15104C4aK. p. subsp. ozaenaeEar pusParis, France1988
    9194C4bK. p. subsp. ozaenaeLungTours, France1989
    9094C4cK. p. subsp. ozaenaeBronchial aspPontoise, France1984
    16114C4dK. p. subsp. ozaenaeBloodCaen, France1989
    7484C4eK. p. subsp. ozaenaeNoseAlgeria1981
    8894C4fK. p. subsp. ozaenaeBronchial aspGrenoble, France1983
    CIP 52.2125C5aK. p. subsp. ozaenaeK5 ref. strainE 5051
    1895C5aK. p. subsp. pneumoniaeLaboratory strain1973
    3255C5aK. p. subsp. pneumoniaeHumanParis, France1975
    4215C5aK. p. subsp. ozaenaeNoseKigali, Rwanda1976
    CIP 52.2136C6aK. p. subsp. pneumoniaeK6 ref. strainF 5052
    CIP 80.426C6bK. p. subsp. pneumoniaeWoodCompiègne, France1979
    SB179, CIP 52.2057C7aK. p. subsp. pneumoniaeK7 ref. strainAerogenes 4140
    15437C7aK. p. subsp. pneumoniaeHorse, penisParis, France1988
    15637C7aK. p. subsp. pneumoniaeHorse, penisParis, France1989
    15837C7aK. p. subsp. pneumoniaeHorseCaen, France1988
    16047C7aK. p. subsp. pneumoniaeHorse, spermParis, France1988
    CIP 52.2068C8aK. planticolaK8 ref. strainKlebsiella 1015
    15748C8bK. p. subsp. pneumoniaeBloodParis, France1988
    SB181, CIP 52.2079C9aK. p. subsp. pneumoniaeK9 ref. strainKlebsiella 1056
    SB182, CIP 52.21410C10aK. p. subsp. pneumoniaeK10 ref. strainKlebsiella 919Source 1eSource 2fDate(s)
    114110C10bK. p. subsp. pneumoniaeBronchial aspLyon, France1986
    CIP 52.21511C11aK. p. subsp. pneumoniaeK11 ref. strainKlebsiella 390
    SB184, CIP 52.21612C12aK. p. subsp. pneumoniaeK12 ref. strainKlebsiella 313
    SB185, CIP 52.21713C13aK. p. subsp. pneumoniaeK13 ref. strainKlebsiella 1470
    SB186, CIP 52.21814C14aK. planticolaK14 ref. strain138
    SB187, CIP 52.21915C15aK. p. subsp. pneumoniaeK15 ref. strainMich. 61
    SB188, CIP 52.22016C16aK. p. subsp. pneumoniaeK16 ref. strain2069/49
    163216C16aK. p. subsp. pneumoniaeSeptic tankParis, France1988
    SB189, CIP 52.22117C17aK. p. subsp. pneumoniaeK17 ref. strain2005/49
    SB190, CIP 52.22218C18aK. p. subsp. pneumoniaeK18 ref. strain1754/49
    SB191, CIP 52.22319C19aK. p. subsp. pneumoniaeK19 ref. strain293/50
    SB19220C20aK. p. subsp. pneumoniaeK20 ref. strain889/50
    SB193, CIP 52.22521C21aK. p. subsp. pneumoniaeK21 ref. strain1702/49
    SB194, CIP 52.22622C22,37aK. p. subsp. pneumoniaeK22 ref. strain1996/49
    SB195, CIP 52.22823C23aK. p. subsp. pneumoniaeK23 ref. strain2812/50
    SB196, CIP 52.22924C24aK. p. subsp. pneumoniaeK24 ref. strain1680/49
    158924C24aK. p. subsp. pneumoniaeBloodParis, France1988
    SB197, CIP 52.23025C25aK. p. subsp. pneumoniaeK25 ref. strain2002/49
    158125C25bK. p. subsp. pneumoniaeBloodParis, France1988
    160125C25bK. p. subsp. pneumoniaeFecesCréteil, France1988
    SB198, CIP 53.626C26aK. oxytocaK26 ref. strain5884
    SB199, CIP 52.23227C27aK. p. subsp. pneumoniaeK27 ref. strain6613
    SB200, CIP 52.23328C28aK. p. subsp. pneumoniaeK28 ref. strain5758
    SB317, CIP 52.23429C29aK. oxytocaK29 ref. strain5725y
    SB318, CIP 52.23530C30aK. p. subsp. pneumoniaeK30 ref. strain7824
    156930C30aK. p. subsp. pneumoniaeHorse, penisParis, France1988
    162330C30aK. p. subsp. pneumoniaeHorse, spermSaint Lo, France1988
    155330C30bK. p. subsp. pneumoniaeHorse, spermLyon, France1989
    SB319, CIP 52.23131C31aK. p. subsp. pneumoniaeK31 ref. strain6258
    SB33932C32aK. planticolaK32 ref. strain6837
    SB473, CIP 53.833C33aK. p. subsp. pneumoniaeK33 ref. strain6168
    SB474, CIP 53.934C34aK. p. subsp. pneumoniaeK34 ref. strain7522
    SB475, CIP 53.1035C35aK. planticolaK35 ref. strain7444
    151635C35bK. p. subsp. pneumoniaeUrineParis, France1988
    138635C35cK. p. subsp. pneumoniaeEnterocolitisMontpellier, France1987
    SB476, CIP 53.1136C36aK. p. subsp. pneumoniaeK36 ref. strain8306
    SB477, CIP 53.1237C22,37aK. p. subsp. pneumoniaeK37 ref. strain8238
    CIP 53.1338C38aK. p. subsp. pneumoniaeK38 ref. strain8414
    SB479, CIP 53.1439C39aK. planticolaK39 ref. strain7749
    58539C39bK. p. subsp. pneumoniaeHandSt Etienne, France1979
    SB480, CIP 53.1540C40aK. p. subsp. pneumoniaeK40 ref. strain8588
    SB48141C41aK. p. subsp. pneumoniaeK41 ref. strain6177
    SB482, CIP 53.1742C42aK. p. subsp. pneumoniaeK42 ref. strain1702
    SB483, CIP 53.1943C43aK. p. subsp. pneumoniaeK43 ref. strain2482
    SB484, CIP 53.2044C44aK. planticolaK44 ref. strain7730
    SB485, CIP 53.2145C45aK. p. subsp. pneumoniaeK45 ref. strain8464
    SB486, CIP 53.2246C46aK. p. subsp. pneumoniaeK46 ref. strain5281
    SB487, CIP 53.2347C47aK. p. subsp. pneumoniaeK47 ref. strain9682
    70047C47aK. p. subsp. pneumoniaeWood, tropicalCompiègne, France1980
    165947C47bK. p. subsp. pneumoniaeFecesParis, France1989
    SB488, CIP 53.2448C48aK. planticolaK48 ref. strain1196
    SB489, CIP 52.19949C49aK. planticolaK49 ref. strain6115
    CIP 52.20050C50aK. p. subsp. pneumoniaeK50 ref. strain1303/50
    SB491, CIP 52.20151C51aK. p. subsp. pneumoniaeK51 ref. strain4715/50
    SB49252C52aK. p. subsp. pneumoniaeK52 ref. strain5759/50
    MGH 7857852C52aK. p. subsp. pneumoniaeReference strain
    157952C52bK. omithinolyticaBronchial aspToulouse, France1988
    SB493, CIP 53.2653C53aK. planticolaK53 ref. strain1756/51
    SB494, CIP 53.2754C54aK. planticolaK54 ref. strainStanley
    SB49555C55aK. p. subsp. pneumoniaeK55 ref. strain3985/51
    SB49656C56aK. planticolaK56 ref. strain3534/51
    SB49757C57aK. planticolaK57 ref. strain4425/51
    SB49858C58aK. planticolaK58 ref. strain636/52
    9459C59aK. oxytocaUnknownParis, France1972
    SB50060C60aK. p. subsp. pneumoniaeK60 ref. strain4463/52
    SB50161C61aK. p. subsp. pneumoniaeK61 ref. strain5710/52
    SB50262C62aK. p. subsp. pneumoniaeK62 ref. strain5711/52
    162962C62aK. p. subsp. pneumoniaeDonkeyMulhouse, France1988
    151362C62bK. p. subsp. pneumoniaeFecesParis, France1988
    19962C62cK. oxytocaBronchial aspParis, France1974
    SB50363C63aK. p. subsp. pneumoniaeK63 ref. strain5845/52
    SB50464C64aK. p. subsp. pneumoniaeK64 ref. strainNCTC 8172
    SB50565C65aK. terrigenaK65 ref. strainSW 4
    SB50666C66aK. oxytocaK66 ref. strain438 (3a)
    SB50767C67aK. p. subsp. pneumoniaeK67 ref. strain264 (1)
    118768C68aK. oxytocaWoundParis, France1986
    134468C68aK. oxytocaBloodBordeaux, France1986
    SB50869C69aK. p. subsp. pneumoniaeK69 ref. strain889
    SB50970C70aK. oxytocaK70 ref. strain167
    SB51071C71aK. planticolaK71 ref. strain4349
    SB51172C72aK. planticolaK72 ref. strain1205
    SB51274C74aK. oxytocaK74 ref. strain371
    SB51379C79aK. planticolaK79 ref. strain325
    SB51480C80aK. p. subsp. pneumoniaeK80 ref. strain708
    SB51581C81aK. p. subsp. pneumoniaeK81 ref. strain370
    SB51682C82aK. p. subsp. pneumoniaeK82 ref. strain3454-70
    10371,58C1aK. p. subsp. pneumoniaeHorsePont l’Eveque, France1985
    10561,58C1aK. p. subsp. pneumoniaeHorseCaen, France1985
    15701,58C1aK. p. subsp. pneumoniaeMare, metritisParis, France1988
    60721,31C21,31aK. p. subsp. pneumoniaeUrineSaint-Etienne, France1978
    162222,37C22,37aK. p. subsp. ozaenaeSputumMali1989
    683AcapsularC2eK. p. subsp. pneumoniaeHumanLyon, France1980
    1340AcapsularC3aK. p. subsp. pneumoniaeBloodBordeaux, France1987
    156730C30aK. p. subsp. pneumoniaeHorse, penisParis, France1988
    1609AcapsularC14aK. p. subsp. pneumoniaeUrineParis, France1988
    SB49959No or weak PCR productK. planticolaK59 ref. strain2212/52
    15862No or weak PCR productK. p. subsp. pneumoniaeBloodBriançon, France1988
    12836No or weak PCR productK. terrigenaCarrotsMulhouse, France1988
    14166No or weak PCR productK. terrigenaHorse, spermNantes, France1988
    1476AcapsularNo or weak PCR productK. p. subsp. pneumoniaeHospital env.Rennes, France1988
    148316No or weak PCR productK. p. subsp. pneumoniaeHospital env.Rennes, France1988
    148524No or weak PCR productK. p. subsp. pneumoniaeHospital env.Rennes, France1988
    15447No or weak PCR productK. p. subsp. pneumoniaeHorse, penisParis, France1988
    157868No or weak PCR productK. oxytocaBloodParis, France1988
    13469No unique PCR productK. p. subsp. pneumoniaeBloodBordeaux, France1987
    15476No unique PCR productK. p. subsp. pneumoniaeMare, uterusCaen, France1988
    15763No unique PCR productK. p. subsp. pneumoniaeUrineParis, France1988
    7754No unique PCR productK. p. subsp. ozaenaeNoseDakar, Senegal1981
Strains in challenge collection of clinical isolatesd
    SB5422, 37C22,37aK. p. subsp. pneumoniaeBloodSeville, Spain1997-1998
    SB5237C22,37aK. p. subsp. pneumoniaeBloodSeville, Spain1997-1998
    SB111124C24aK. p. subsp. pneumoniaeUrineCoimbra, Portugal1997-1998
    SB6425C25bK. p. subsp. pneumoniaeBloodLondon, United Kingdom1997-1998
    SB11827C27aK. p. subsp. pneumoniaeWoundAthens, Greece1997-1998
    SB13827C27aK. p. subsp. pneumoniaeWoundAthens, Greece1997-1998
    SB15330C30aK. p. subsp. pneumoniaeBloodAnkara, Turkey1997-1998
    SB16230C30aK. p. subsp. pneumoniaeBloodIstanbul, Turkey1997-1998
    SB22330C30aK. p. subsp. pneumoniaeBloodAnkara, Turkey1997-1998
    SB11430C30bK. p. subsp. pneumoniaeBloodAthens, Greece1997-1998
    SB59230C30bK. p. subsp. pneumoniaeBloodAthens, Greece1997-1998
    SB9339C39bK. p. subsp. pneumoniaeBloodKrakow, Poland1997-1998
    SB21052C52aK. p. subsp. pneumoniaeUrineLille, France1997-1998
    SB4462C62aK. p. subsp. pneumoniaeBloodUtrecht, The Netherlands1997-1998
    SB16435C-NEW-1K. p. subsp. pneumoniaeBloodAthens, Greece1997-1998
    SB20422, 37CNEW-2K. p. subsp. pneumoniaeBloodLondon, United Kingdom1997-1998
    SB120NTCNEW-3K. p. subsp. pneumoniaeBloodGenoa, Italy1997-1998
    SB39NTCNEW-3K. p. subsp. pneumoniaeBloodGenoa, Italy1997-1998
    SB11324C24alike-aK. p. subsp. pneumoniaeUrineLille, France1997-1998
    SB15868C68alike-aK. p. subsp. pneumoniaeUrineGenoa, Italy1997-1998
    03-91382C2aK. p. subsp. pneumoniaeAbcessFrance2003
    03-24553C3aK. p. subsp. rhinoscleromatisNoseMali2003
  • a Entries with two strain names separated by a comma correspond to the 47 duplicates (see Materials and Methods).

  • b NT, nontypeable; two numbers separated by a comma mean that the strain reacted with both antisera.

  • c K. p., K. pneumoniae.

  • d The K serotypes of the clinical strains were determined in this study after cps PCR-RFLP analysis.

  • e ref., reference; CSF, cerebrospinal fluid; asp, aspirate; env., environment.

  • f Where source 1 is a reference strain, source 2 is the original strain name in the reference collection of the WHO reference center for E. coli and Klebsiella.