TABLE 2.

Antibody neutralization titers determined by FACS and PRNT assaysa

SerumFNT50FNT80PRNT50PRNT80
Normal human serum<20<20<20<20
H816310,2402,5605,1201,280
H7859<20<20<20<20
  • a Vero cells were infected with DENV2 NGC that had been incubated with serial two-fold dilutions of serum from two human patients previously infected with dengue virus. The FNT and PRNT were analyzed at 24 hpi and 6 days postinfection, respectively. The 50% and 80% neutralization titers were determined based on the percent reduction observed by each method. H8163 was from a secondary infection, and H7859 was from a primary infection, serotype unknown.