TABLE 1.

Characteristics of oligonucleotides used in the multiplex PCR assay

Target genePrimer sequence (5′→3′)Concn (nM)Amplicon length (bp)Repeat length (bp)Source or reference
Expected in MW2Measured
mecA F-CATTGATCGCAACGTTCAATTT200
R-TGGTCTTTCTGCATTCCTGGA2009999 ± 1NoThis study
sspA F-ATCMATTTYGCMAAYGATGACCA400
R-TTGTCTGAATTATTGTTATCGCC400133132 ± 224 31
spa F-AGCACCAAAAGAGGAAGACAA400
R-GTTTAACGACATGTACTCCGT400284290 ± 49 31
sdrCDE F-TTACGGATCATGATGATTTCA300
R-CAYTACCTGTTTCTGGTAATGCTT300648/554/606664/580/62218This study
clfA F-GATTCTGACCCAGGTTCAGA300
R-CTGTATCTGGTAATGGTTCTTT3001057945 ± 2218 31
clfB F-ATGGTGATTCAGCAGTAAATCC300
R-CATTATTTGGTGGTGTAACTCTT300918880 ± 1918 31
fnBP F-GGTCAAGCRCAAGGACCART200
R-AATAATCCGCCGAACAACAT20010911045 ± 3240This study
cna F-AAAATGACAAAAATGGCAAG400
R-CAGGTTTAGTTGGTGGTGTT40018781888 ± 125560This study