TABLE 1.

HIV-1 RNA loads in spiked plasma samples for three dilutions of HIV-1, measured in duplicate by a Biocentric kit after RNA extraction with four RNA extraction kits

Extraction kit (manufacturer)Mean plasma log10 copies/ml ± SD (log difference compared to Qiagen kit) at indicated dilution
3.30 ± 0.404.31 ± 0.115.32 ± 0.15
QIAamp viral RNA mini kit (Qiagen)3.38 ± 0.024.44 ± 0.125.42 ± 0.02
Sample preparation system (Abbott)3.40 ± 0.12 (−0.02)4.20 ± 0.25 (0.25)5.10 ± 0.15 (0.31)
Nuclisens manual extraction kit (bioMérieux)3.69 ± 0.06 (−0.31)4.34 ± 0.17 (0.11)5.42 ± 0.06 (−0.01)
High Pure viral nucleic acid kit (Roche)2.74 ± 0.24 (0.64)4.26 ± 0.34 (0.19)5.33 ± 0.19 (0.09)