TABLE 3.

Amplification of RT and PR regions of the HIV-1 pol gene from DPS prepared with three dilutions of spiked HIV-1 and tested in duplicate after RNA extraction with four RNA extraction kits

Dilution (log10 copies/ml)Region amplifiedAmplification with RNA extraction kita
QIAamp viral RNA mini kit (Qiagen)Sample preparation system (Abbott)Nuclisens manual extraction kit (bioMérieux)High Pure viral nucleic acid kit (Roche)
3.30RT + +
+ +
PR +
+ +
4.31RT + +
+ + +
PR + +
+ + +
5.32RT + + + +
+ + +
PR + + + +
+ + +
  • a −, no amplification; +, amplification.