TABLE 2.

HIV-1 viral loads in DPS after RNA extraction with four different RNA extraction kits

Spiked plasma dilutiona (log10 copies/ml)DPS extracted by QIAamp viral RNA mini kit (Qiagen)bDPS extracted by Abbott sample preparation systemDPS extracted by Nuclisens manual extraction kit (bioMérieux)bDPS extracted by High Pure viral nucleic acid kit (Roche)b
VLMeanPlasma − DPScVLMeanPlasma − DPScVLMeanPlasma − DPScVLMeanPlasma − DPSc
3.302.852.880.423.250.05
2.68NA0.623.152.62
2.773.57
2.763.56
4.312.832.891.423.583.610.704.224.060.24
2.953.643.912.63NA1.68
5.323.883.821.504.544.580.745.395.33−0.013.473.282.04
3.984.484.953.71
3.584.675.472.68
3.844.645.513.25
  • a DPS were prepared with three different dilutions of human HIV-negative plasma spiked with HIV-1 (Table 1) and were tested in duplicate, and the samples with the lowest and highest viral loads were tested twice in duplicate.

  • b —, undetectable; NA, not applicable.

  • c Difference in log10 copies/ml between the viral loads in plasma and DPS.