TABLE 5.

Amplification of RT and PR regions of the HIV-1 pol gene from each DPS, in duplicate, for three dilutions of spiked HIV-1 after RNA extraction over time and at different ambient temperatures and conditions

Plasma viral load (log10 copies/ml)Extraction kitAmplification of indicated regionb
Time 0a1 wk2 wk1 mo2 mo3 mo at 20°C
20°C37°C20°C37°C20°C37°C20°C37°C
RTPRRTPRRTPRRTPRRTPRRTPRRTPRRTPRRTPRRTPR
3.30Sample preparation system (Abbott) + + +
Sample preparation system (Abbott) + + + + + NDND
Nuclisens manual extraction kit (bioMérieux) + + + + + +
Nuclisens manual extraction kit (bioMérieux) + + + + + + + +
4.31Sample preparation system (Abbott) + + + + + + + + + + +
Sample preparation system (Abbott) + + + + + + + + + + +
Nuclisens manual extraction kit (bioMérieux) + + + + + + + + + + + + + +
Nuclisens manual extraction kit (bioMérieux) + + + + + + + + + + + + + +
5.32Sample preparation system (Abbott) + + + + + + + + + + + + +
Sample preparation system (Abbott) + + + + + + + + + + + + + +
Nuclisens manual extraction kit (bioMérieux) + + + + + + + + + + + + + + + +
Nuclisens manual extraction kit (bioMérieux) + + + + + + + + + + + + + + + +
  • a Results are from Table 3.

  • b +, amplification; −, no amplification; ND, not done.