TABLE 4.

Ribotyping, GeneChip, and PFGE genotyping results

StrainGenotyping resulta
GeneChipRibotypePFGE
CDC 11.1XIIUSA300 (0.0114)
CDC 31.1XIIUSA300 (0.0114)
CDC 41.1XIIUSA300 (0.0114)
CDC 61.1XIIUSA300 (0.0114)
CDC 51.1XIIUSA300 (0.0114)
CDC 21.2XIIUSA300 (0.0114)
CDC 191.3XIIUSA500 TYPE (0.0004)
NCTC 83251.4XIIIND
COL (lab 1)1.5IXND
COL (lab 2)1.5NDND
COL (repository 1)1.5NDND
COL (lab 3)1.5NDND
CDC 102.1XIUSA400 (0.0051)
CDC 132.1XIUSA400 (0.0051)
CDC 122.2XIUSA400 (0.0051)
CDC 92.2XIUSA400 (0.0051)
MW22.3XIND
CDC 72.4IVUSA400 (0.0199)
CDC 82.5XIUSA400 (0.0051)
CDC 142.6XUSA400 (0.0172)
MSSA4762.7XIND
CDC 112.8XIUSA400 (0.0080)
CDC 212.9VIUSA700 TYPE (0.0097)
CDC 163.1VUSA100/800
N3153.2NDND
COL (repository 2)3.3NDND
CDC 203.4IIUSA600 TYPE
CDC 173.5VIIUSA100-B (0.0022)
Mu50 (1)3.6NDND
Mu50 (2)3.6NDND
CDC 154.1IIIUSA600 (0.0121)
CDC 184.2VIIIUSA200 TYPE
MRSA2524.3IND
  • a Ribotyping, GeneChip, and PFGE results are shown for each strain. Strains were observed to fit into four major clusters (1 to 4) by GeneChip analysis (Fig. 3A). Individual strains within each of these clusters were further distinguished. For example, GeneChip profiles 2.2 and 2.3 are different strains within cluster number 2. Strains with the same profile numbers are identical. Ribotyping results distinguished strains as belonging to one of 12 different ribogroups (I to XII). PFGE results demonstrated that strains belonged to eight different groups (USA100 to USA800; 80% identity cutoff). Numbers in parentheses represent the strain's identification number. Strains with the same identification number are considered identical. ND, not determined.