TABLE 1.

Serology results and genetic characterization of Bartonella spp. detected in two Greek dogs and seven Italian dogs, based upon DNA amplification and sequencing of three distinct portions of the genomea

Dog no.Country/regionSerology titer by IFABartonella species/strain DNA amplification by PCR of the:
B. henselaeB. vinsonii subsp. berkhoffiiITS region16S rRNA generpoB gene
1Greece/ThessalonikiNPNP B. rochalimae B. rochalimae B. rochalimae
2Greece/ThessalonikiNPNPHMDHMD-1/HMD-2HMD
3Italy/Basilicata64<16HMDHMD-3HMD
4Italy/Basilicata12832HMDHMD-3HMD
5Italy/Basilicata256<16HMD and B. vinsonii subsp. berkhoffii genotype III B. vinsonii subsp. berkhoffii genotype IIIHMD and B. vinsonii subsp. berkhoffii genotype III
6Italy/Basilicata6432HMDNPHMD
7Italy/Basilicata<16<16HMDNPHMD
8Italy/Basilicata<1664HMDHMD-3HMD
9Italy/GinosaNPNP B. vinsonii subsp. berkhoffii genotype IINPNP
  • a IFA, microimmunofluorescence test; NP, not performed; HMD, Bartonella sp. strain HMD.