TABLE 1.

Summary of genotypic and phenotypic characterization results obtained in this study for cpb2-positive C. perfringens isolates from horses with GI diseases

StrainBeta2-toxin-specific Western blotting resultsacpb2 RFLP pattern (kb) with HpaIcpb2 location determined by PFGEbNorthern blotting resultscReference
cpb2-positive CWC245+∼5Plasmid+ 11
cpb2-negative 106527 11
Diarrheic-horse isolates
    106888>20Plasmid+ 13
    106889>20Plasmid+ 13
    106902>20Plasmid+ 13
    106903>20Plasmid+ 13
    100>20ND+ 13
    101>20ND+ 13
    170>20ND+ 13
    1991>20ND+ 13
    1992>20ND+ 13
    1993>20NDND 13
    1994>20ND+ 13
    1995>20ND+ 13
    1996>20NDND 13
    AHT327>20ND+ 10
    AHT2600∼8ND+ 10
    AHT2779∼14ND+ 10
    AHT2911>20ND+ 10
    AHT3553>20ND+ 10
    AHT3992>20Plasmid+ 10
    AHT4578>20Plasmid+ 10
    AHT5195>20NDND 10
    AHT5773>20Plasmid+ 10
    AHT45771>20NDND 10
  • a +, presence of 28-kDa CPB2 immunoreactive band; −, absence of 28-kDa CPB2 immunoreactive band.

  • b ND, not determined.

  • c +, presence of ∼1.2-kb cpb2 transcript; −, absence of ∼1.2-kb cpb2 transcript.