TABLE 2.

Detection of rpoB gene mutations of M. tuberculosis in Wuhan by SOLAC compared with DNA sequencing results

Number of strainsRifampin resistanceaMutationsb
SOLAC assayDNA sequencing
26R531 TCG→TTG531 TCG→TTG
3R531 TCG→TGG531 TCG→TTG
7R526 CAC→TAC526 CAC→TAC
3R526 CAC→GAC526 CAC→GAC
3R526 CAC→CGC526 CAC→CGC
2R516 GAC→TAC516 GAC→TAC
3R516 GAC→GTC516 GAC→GTC
1R531 TCG→TTG531 TCG→TTG, 511 CTG→TTG
1R526 negativec526 CAC→AC, one base deletion
1RWT511 CTG→CGG
2RWT513 CAA→CTA
1RWT533 CTG→CCG
3RWTWT
1 (H37Rv)SWTWT
5SWTWT
  • a R, resistant; S, sensitive.

  • b WT, wild type.

  • c Negative, no results of ligation could be seen.