TABLE 1.

Probes designed to detect mutations in the rpoB gene

Amino acid position and probeaSizeGroupSequence (5′→3′)b
516
    516Com19Biotin-GTTGTTCTG
    516Com215Biotin-CAGCGGGTTGTTCTG
    516Wt1516pGTCCATTTTTTTTTT
    516A11516pGACCATTTTTTTTTT
    516A21631pGTACAATTTTTTTTTT
    516A31516pGCCCATTTTTTTTTT
    516A41516pCACCATTTTTTTTTT
526
    526Com15Biotin-CGACAGTCGGCGCTT
    526Wt1526pGTGGGATTTTTTTTTT
    526A11526pGTAGGTTTTTTTTTTT
    526A21526pGTCGGTTTTTTTTTTT
    526A31626pGTTGGTTTTTTTTTTT
    526A41626pGAGGGTTTTTTTTTTT
    526A51626pGCGGGTTTTTTTTTTT
    526A61626pTTGGGTTTTTTTTTTT
    526A71626pCTGGGTTTTTTTTTTT
    526Ex15TCAACCCCGACAGCG
531
    531Com15Biotin-CCCCAGCGC
    531Wt1531pCGACATTTTTTTTTT
    531A11531pCAACATTTTTTTTTT
    531A21531pCCACATTTTTTTTTT
    531A31531pCGGCATTTTTTTTTT
    531A41631pAGACAGTTTTTTTTTT
    531Ex111AGACCGCCGGG
    531Ex215CGCTTGTGGGTCAA
  • a Com, common probe; Wt, wild-type probe; A, allele-specific probe; Ex, extra probe.

  • b The italic “T” was modified (CH2)6-S-S-(CH2)6-(PO4) on the 3′ end. The common probes contained biotin labels on their 5′ ends. The allele-specific probes contained 5′-phosphate groups and the discriminating bases near their 5′ ends (in bold). Subscript p, 5′-phosphate group.