TABLE 2.

ORFs in and around φ2958PVL and their similarities to φSa2mw and φ108PVLa

ORF and special structureStart (bp)End (bp)Size (bp)aaaGeneFunctionφSa2mwφ108PVL
% Identity to φSa2mw genomeCorresponding ORF (size in bp)% aa identity% Identity to φ108PVL genomeCorresponding ORF (size in bp)% aa identity
(JP001)b295936642214Hypothetical protein
attB184286625Attachment site on chromosome
Core86789529Core sequence
attP-left89692025Attachment site on phage
JP00221849791,206401intPhage integrate98.7Integrase (1,206)99.898.6Integrase (1,206)99.8
JP00323102924615204Na/K ATPase47.4Putative membrane protein MW1441 (624)26.752.1
JP0043285310318360Hypothetical protein52.252.2
JP00543013381921306Hypothetical protein45.945
JP00649314317615204repPutative transcriptional repressor45.2Repressor (324)34.349.1cI-like repressor (717)24
JP0075103533022875Hypothetical protein54.454.7Putative cro-like repressor (243)23.1
JP0085356552016554antPutative antirepressor52.150.3Putative antirepressor (753)31.4
JP0095927614521972Hypothetical protein51.852
JP0106195644024681Hypothetical protein100MW1434 (246)10047.9
JP01167746409366121Hypothetical protein100MW1433 (366)10046.1
JP0126829704421671Hypothetical protein77.3MW1432 (216)66.255.6
JP0137069733226487Hypothetical protein82.6MW1431 (264)78.899.6Putative DNA-binding protein (264)98.9
JP0147347750515952Hypothetical protein93.7MW1430 (162)96.298.7
JP01575847907324107Hypothetical protein92.9MW1429 (324)91.651
JP01679228284363120Hypothetical protein97.8MW1428 (363)97.548.9
JP017828194471,167388Hypothetical protein97.4MW1427 (1,167)97.946.5
JP018947310030558185Hypothetical protein98.7MW1426 (558)99.549.7
JP01910098120501,953650polDNA polymerase99.8DNA polymerase (1,962)99.747.5
JP020120631224818661Hypothetical protein100MW1424 (186)10069.9
JP0211224512649405134Putative DNA-binding protein100MW1423 (402)10057
JP022126491290625885Hypothetical protein97.7MW1422 (258)97.652.8
JP023131181336624982Hypothetical protein92.4MW1420 (243)93.891.6P028 (249)89
JP024134071366125584Hypothetical protein89.8MW1419 (255)86.696.8P030 (372)98.8
JP0251381114347537178Hypothetical protein95.2MW1415 (534)96.649.1
JP026146451488123778Hypothetical protein54100P032 (237)100
JP027148651502616253Hypothetical protein91.7MW1411 (156)86.392.1
JP028150941529420166Hypothetical protein98.5MW1410 (201)9761.6
JP02915347177942,448815virEVirulencc-associated protein E98.9Virulence-associated protein E (2,448)99.646.7
JP030181351842529196Hypothetical protein95.2MW1406 (198)98.550
JP03118502197731,272423Phage helicase99.4Phage helicase (1,359)10047.8
JP0321978620223438145Phage regulatory protein89.7MW1404 (438)93.851.4
JP0332038020694315105Hypothetical protein97.5MW1403 (315)95.251.5
JP0342080521128324107terSTerminase small subunit97.5Terminase small subunit (306)10049.4Terminase small subunit (468)28.6
JP03521448228091,362453terLTerminase large subunit91.6Terminase large subunit (1,692)96.546.1Terminase large subunit (1,695)24.8
JP03623006240521,047348porPortal protein98.9Portal protein (1,239)98.948.7Portal protein (1,326)21.9
JP0372403624809774257proProhead protease99.7Protease (774)10046.7Prohead protease (585)24.6
JP03824776259841,209402Putative capsid protein99.9Capsid (1,164)10049.3Capsid (1,248)19.2
JP039260532633127992Hypothetical protein100MW1397 (279)10049.4
JP0402634326675333110Hypothetical protein96.4MW1396 (333)97.349.5
JP0412667227073402133Hypothetical protein99.5MW1395 (402)10048.5
JP0422707427469396131Hypothetical protein100MW1394 (396)10050.4
JP0432750428145642213Major tail protein99.8Major tail protein (642)10047.8
JP0442823728692456151Major tail protein99.8Major tail protein (456)99.349.3
JP0452875029100351116Hypothetical protein99.7MW1391 (342)10050.4
JP046291422930015952Hypothetical protein100No corresponding gene56.8
JP04729314355146,2012066mtpTail length tape measure protein98.1Tail length tape measure protein (6,201)98.946.4Tail length tape measure protein (3,333)25.3
JP0483551436338825274Hypothetical protein100MW1389 (825)10048.5
JP04936347379301,584527Hypothetical protein100MW1388 (1,584)10045.7
JP050379303822029196Hypothetical protein100MW1387 (291)10049.5
JP05138236401461,911636Hypothetical protein99.6MW1386 (1,911)99.845.4
JP05240113416121,500499Hypothetical protein99.7MW1385 (1,467)99.847.5
JP0534161242001390129Hypothetical protein100MW1384 (390)10048.1
JP054419944215816554Hypothetical protein100MW1383 (165)10054
JP055422044250330099Hypothetical protein100MW1382 (300)10051.1
JP0564263942941303100holHolin100Holin (303)100100Holin (303)100
JP05742952444061,455484amlAmidase (peptidoglycan hydrolase)98.3Amidase (1,455)99.298.3Amidase (1,455)99.2
JP058445564468713243Hypothetical protein98.5No corresponding gene98.5
JP0594479545733939315lukS-PVLukS-PV99.8LukS-PV (939)99.7100LukS-PV (939)100
JP0604573546712978325lukF-PVLukF-PV99.9LukF-PV (978)10099.9LukF-PV (978)100
attP-right469174694125Attachment site on phage
Core469424697029Core sequence
attB2469714699525Attachment site on chromosome
  • a aa, amino acid.

  • b ORF in parentheses was located outside of the φ2958PVL. Two sets of core sequences and attachment site sequences were also identified flanking the φ2958PVL.