TABLE 4.

Calculated efficiencies of each multiplex or monoplex PCR assay with nucleic acids from single viruses and mixtures of viruses

PCR type and nucleic acid source(s)Efficiency (R2 value) of PCR assay for:
NoV GIIRVEAVNoV GIEAVSaVAsVAdV
Multiplex PCR
    NoV GII, RV, EAV1.000.991.00
    NoV GII, RV1.000.99
    NoV GII, EAV1.001.00
    RV, EAV1.000.98
    NoV GII0.98
    RV0.89
    EAV0.9975
    NoV GI, EAV0.990.99
    NoV GI0.99
    EAV0.99
    AsV, SaV, AdV1.000.990.97
    AsV, SaV1.001.00
    AdV, SaV1.000.99
    AsV, AdV0.980.99
    SaV0.99
    AsV0.98
    AdV1.00
Monoplex PCR
    NoV GII, RV, EAV1.000.960.99
    NoV GII, RV0.960.99
    NoV GII, EAV1.000.99
    RV, EAV1.000.99
    NoV GII0.91
    RV0.98
    EAV1.00
    NoV GI, EAV1.000.99
    NoV GI1.00
    EAV0.99
    AsV, SaV, AdV0.990.990.97
    AsV, SaV1.001.00
    AdV, SaV1.000.99
    AsV, AdV0.990.99
    SaV1.00
    AsV0.99
    AdV0.98