TABLE 3.

Comparison of results of mPCR/RLB and comparator methods in detection of genital infection/colonization with 14 recognized or putative genital pathogens

OrganismLimit of detectiona (ng/μl)No. positively detected by mPCR/RLBbComparator methodc (target)No. positively detected by comparator method
T. vaginalis1.5 × 10−91sPCR (18S rRNA gene)1
S. pneumoniae7.4 × 10−11Culture1
N. gonorrhoeae4.3 × 10−27Culture and Amplicor PCR2 (+5)d
C. trachomatis7.0 × 10−1155Amplicor PCR50 (+5)d
Ureaplasma spp.e7.8 × 10−984Culture86
G. vaginalis7.8 × 10−33sPCR (16-23S rRNA gene)3
H. influenzae7.0 × 10−831Culture35
HSV14.3 × 10−88sPCR (pol)5
N. meningitides8.2 × 10−82Culture1
M. hominis5.5 × 10−115Culture16
M. genitalium2.1 × 10−915sPCR (16S rRNA gene)15
Adenovirus6.3 × 10−74sPCR (hexon gene)4
  • a For mPCR/RLB.

  • b sPCR, using the same primers as those for mPCR, was performed on specimens with discrepant mPCR/RLB and culture results. In all cases the mPCR/RLB and sPCR results were concordant.

  • c Comparator methods were either the culture of urethral swab collected at the same time as first-voided urine specimen or sPCR on the same urine DNA extract as that used for mPCR/RLB, using a different, species-specific target (except for adenoviruses, for which the same hexon gene target was used).

  • d Of the 7 and 55 specimens positive by mPCR/RLB for N. gonorrhoeae and C. trachomatis, respectively, only 2 and 50 were positive initially in the Roche Amplicor PCR; all were positive on retesting.

  • e Urethral specimens were cultured for ureaplasmas, but isolates were not speciated. Ureaplasma spp. identified in the mPCR/RLB-positive specimens are shown in Fig. 2.