TABLE 1.

Oligonucleotide primers and probes developed or modified for the mPCR/RLB assay used in this studya

Primer/probe namebSpecificityc (target)GenBank accession no.Primer-probe sequenced (5′-3′)Tme (°C)Reference or sourceg
TV-ApT. vaginalis (btuB)904TGT TGT GAG CTT GAG TGT ACG G88365.1084This study
TV-Sp916CGA TCT TAA CCA CCT TGT TTC C94563.3192314 (mod)
NGpSbN. gonorrhoeae (CP)M103163249TGC TGT TTC AAG TCG TCC AG326864.06359This study
NGpAp3317GAT AGT CAT AGC AGG GCT GTT C329661.5549This study
NGpSp3452CCG TAA CGT CTC TAA GTC TGC TT347462.51156This study
NGpAb3503CGA AGC CGC CAG CAT AGA GC348471.22094This study
NG16SbN. gonorrhoeae (ITS)AF223396404CCA AAA CTT AAC AAA TGA AAG CAA G42863.41This study
NG1S453TGA TTT GCG AAG TAG AAT AAC G47460.64This study
NG2A2456ATC AAA ATA AGC TGC TAA AAA CAG43359.41This study
NGITSAb490TGT TAA AGA TCG ATG CGT CGT47264.33This study
CT24bC. trachomatis (CP)X06707840GGG ATT CCT GTA ACA ACA AGT CAG G86467.33045This study
CTS1p865TTG CGC ATA ATT TTA GGC TTG88563.59178This study
CTA2p1021ACA CTT TGT CTC GAT GAA AGA CA99962.57137This study
CT27b1047CCT CTT CCC CAG AAC AAT AAG AAC AC102267.37814This study
UP-SbU. parvum (ureB)AF085731637GAT CAC ATT TTC ACT TGT TTG AAG TG66264.3776723 (mod)
UP-Ap702CTT CAT TTC CTT TTT CAT CAA AAA ATA C67563.43446This study
UP-Sp688AAA AAG GAA ATG AAG ATA AAG AAC G71261.4032223 (mod)
UP-Ab735AAC GTC GTC CAT AAG CAA CTT TG71365.887523 (mod)
UU-ApU. urealyticum (ureB)705CTT CAT TTC CTT TTT CAT CAA AAA ATA C67863.43446This study
UU-Sp691AAA AAG GAA ATG AAG ATA AAG AAC G71561.4032223 (mod)
UU-Ab739AAA CGA CGT CCA TAA GCA ACT TTA71664.3431723 (mod)
GV-ApG. vaginalis (ITS)416TCC TGT CTA CCA AGG CAT CC39763.97078This study
GV-Sp632CGT GTG ATA ACC GTC AGG TG65164.06355This study
HSV1-SpHSV1 (gD)545CGT TTG AGA CCG CCG GCA56273.04224This study
HSV2-Sp572CCT TCG AGA CCG CGG GTA58968.53278This study
NM-SbN. meningiditis (porA)AY319969929GCT TCG GTA ATG CAG TTC CA94864.9424217 (mod)
NM-Ap1010CTG GTA TTT TCG CCT TTT TTA C98917 (mod)
NM-Sp953TCA GCT ATG CCC ATG GTT970This study
MH-ApM. hominis (gap)770CTG AAT AAA CAA CTG TTT TAA CAC CTT CGCT74068.70162 (mod)
MH-Sp702CAG GTG CTA AAA AGG TGT TTA TTA CTG CT73066.55416This study
MgPAa-ApM. genitalium (mgpA)1463TAT CAT ACC TTC TGA TTG CAA AGT144560.39573This study
MgPa-Sp1473CGG TAG AGC TTT ATA TGA TAT TAA CTT AGC150261.46476This study
AdVdeSbAdenovirus (hexon)GCC SCA RTG GKC WTA CAT GCA CAT Cf69.21 (mod)
AdVdeApCCY ACR GCC AGI GTR WAI CGM RCY TTG TA68.163711 (mod)
AdVdeSpGCC CGY GCM ACI GAI ACS TAC TTC63.766651 (mod)
AdVdeAbCAG CAC SCC ICG RAT GTC AAA62.802431 (mod)
  • a In addition to primers specifically designed or modified for this study, published primers were used without modification for several targets. Details are shown in the supplemental material.

  • b The suffix b indicates a biotin-labeled primer, and p indicates an amine-labeled probe. An A indicates antisense, and S indicates sense.

  • c Abbreviations: CP, cryptic plasmid; ITS region, intergenic spacer region; gD, glycoprotein D.

  • d Numbers represent the base positions at which the primer/probe sequence starts and finishes (starting at point 1 of the corresponding gene sequence in GenBank).

  • e Melting temperatures were provided by the primer synthesizer (Sigma-Aldrich).

  • f S = G+C; R = A+G; K = G+T; W = A+T; Y = C+T; M = A+C; I = inosine.

  • g Some primers were modified (mod) from the published primers.