TABLE 2.

Oligonucleotide primers used for sPCR

Primer nameSpecificity (target)GenBank accession no.Primer sequencea (5′-3′)Tmb (°C)Reference
Tv1T. vaginalis 18S rRNA geneU17510874TAA TGG CAG AAT CTT TGG AG89459.225
Tv21185GAA CTT TAA CCG AAG GAC TTC116558.3
HSVPolA1HSV DNA polymeraseATC ATC TAC CGC GAC ACG GACT68.849
HSVPolA2TCC ACG CCC TTG ATG AGC ATC T72.0
MG16-45FM. genitalium 16S rRNA geneX7733445TAC ATG CAA GTC GAT CGG AAG TAG C6968.816
MG16-447R469AAA CTC CAG CCA TTG CCT GCT AG44769.2
AD1Adenovirus hexon geneU20821CTG ATG TAC TAC AAC AGC ACT GGC AAC ATG GG76.136
AD2GCG TTG CGG TGG TGG TTA AAT GGG TTT ACG TTG TCC AT83.4
GV1G. vaginalisLO8167364TTA CTG GTG TAT CAC TGT AAG G38555.851
16S-23S rRNA gene
GV223S695CCG TCA CAG GCT GAA CAG T67764.1
  • a Numbers represent the base positions at which the primer/probe sequence starts and finishes (starting at point 1 of the corresponding gene sequence in GenBank).

  • b Melting temperatures were provided by the primer synthesizer (Sigma-Aldrich).